Anti-Human CD225/IFITM1 Monoclonal Antibody for Flow Cytometry (Intracellular) - P13164

Core Product Specifications and Parameters

Biological Background: IFITM1 Function and Localization

• IFITM1 is an interferon-induced transmembrane protein that belongs to the dispanin family (subfamily A member 2a) and is also known as CD225, Leu-13, or 9-27.
• Functions as a broad-spectrum antiviral restriction factor by blocking viral entry at the endosomal/lysosomal stage; it permits endocytosis but prevents viral fusion, thereby inhibiting viruses such as influenza A, SARS-CoV-1, SARS-CoV-2, Ebola, Marburg, HIV-1, HCV, Dengue, and West Nile virus. Related references: PMID:26354436, PMID:33270927
• Inhibits SARS-CoV-2 spike-mediated syncytia formation, contributing to restriction of cell-cell fusion. Related references: PMID:33051876
• In hepatocytes, IFITM1 coordinates with IFITM2/3 to target HCV virions for lysosomal degradation. Related references: PMID:26354436
• Localizes to the cell membrane and lysosome membrane, consistent with its role in viral entry restriction.
• Additional roles: IFN-gamma-induced antiproliferative effects via ERK inhibition or p53-dependent G1 arrest; positive regulation of osteoblast differentiation; and clinical relevance in cancer, with elevated expression in colon, cervical, esophageal, ovarian cancers, and gliomas.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 1-80 amino acids, which includes the extracellular domain; consider this when designing blocking peptides or epitope mapping experiments.
• Since this antibody is validated for intracellular flow cytometry, ensure appropriate permeabilization when detecting IFITM1, as the protein localizes to the cell membrane and endosomal/lysosomal compartments.
• IFITM1 expression is interferon-inducible; consider stimulating cells with IFN-α/γ to upregulate the target in your experimental system.
• Given its role in viral entry, this antibody may be useful for studying viral restriction mechanisms in cell culture models.

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CamelBio offers a seamless sourcing experience for IVD raw materials, connecting researchers and manufacturers with high-quality reagents like this anti-IFITM1 monoclonal antibody. Whether you need validated antibody pairs, bulk ancillary reagents, or assistance in sourcing rare-target raw materials for antiviral or oncology diagnostics, our one-stop platform accelerates your development from concept to clinic.