Anti-Human CD2 Monoclonal Antibody () for Flow Cytometry - P06729

Core Product Specifications and Parameters

Biological Background: CD2 Function and Localization

• CD2 (T-cell surface antigen CD2) is a glycoprotein of the immunoglobulin superfamily, also designated LFA-2, T11, SRBC receptor, or Leu-5.
• It mediates cell adhesion by binding to ligands CD58 (LFA-3) and CD48, facilitating T-cell interactions with antigen-presenting cells, target cells, and other lymphocytes.
• The CD2–CD58 interaction strengthens the immunological synapse, contributing to T-cell activation, costimulation, and signal transduction.
• CD2 is expressed on T lymphocytes and natural killer (NK) cells, where it functions in both adhesion and intracellular signaling.
• Its cytoplasmic domain links to intracellular kinases and adaptor proteins, playing a critical role in T-cell triggering.
• Structurally, CD2 comprises two extracellular Ig-like domains, a single-pass transmembrane helix, and a short cytoplasmic tail containing signaling motifs.
• Subcellularly, CD2 localizes to the plasma membrane and contains disulfide bonds and N-linked glycosylation sites.
• Key post-translational modifications include glycosylation; the protein forms rosettes with sheep erythrocytes via its SRBC receptor activity.

Experimental Guidance and Technical Tips

• The immunogen used spans the extracellular domain (amino acids 25–209), targeting the ligand-binding region; thus the antibody is expected to recognize surface CD2.
• As an direct conjugate, this antibody is optimized for flow cytometry on live or fixed cells without the need for secondary reagents.
• Titrate the antibody (e.g., 0.1–5 µg per test) to determine the optimal staining concentration for your experimental system.
• Validate staining in human PBMCs or isolated T/NK cell populations; use appropriate isotype controls to set gates.
• For multicolor panels, check spectral compatibility of (excitation 491 nm, emission 518 nm) with other fluorophores.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive procurement solutions for IVD raw materials, supporting diagnostic developers and research laboratories with validated antibodies, bulk reagents, and rare target sourcing. For T-cell immunophenotyping and CD2-related studies, we provide directly conjugated monoclonal antibodies, such as this anti-human CD2 clone, along with ancillary reagents and custom supply options. Let us streamline your raw-material supply chain from discovery to clinical application.