Anti-Human CD2 Monoclonal Antibody () for Flow Cytometry - P06729

Core Product Specifications and Parameters

Biological Background: CD2 Function and Localization

• CD2 (T11, SRBC, LFA-2) is a cell adhesion molecule found on the surface of T lymphocytes and natural killer cells, where it functions as a coreceptor in T-cell activation.
• It mediates intercellular adhesion by binding to its ligands CD58 (LFA-3) and CD48, facilitating the formation of immunological synapses.
• The cytoplasmic tail of CD2 is critical for signal transduction, linking extracellular binding events to intracellular signaling cascades upon T-cell stimulation.
• CD2 is a transmembrane glycoprotein containing two extracellular immunoglobulin-like domains (V-type and C2-type) and a single transmembrane helix.
• While primarily expressed on T cells and NK cells, CD2 is also detected on some other leukocyte subsets, and its expression can be modulated during immune responses.
• Post-translational modifications include N-linked glycosylation, and its molecular weight is approximately 39-40 kDa.
• CD2 is the receptor for the sheep erythrocyte rosette formation, a classic assay for human T cells (hence the alias SRBC receptor).

Experimental Guidance and Technical Tips

• Titrate the antibody to determine the optimal concentration for your specific cell type and experimental setup.
• Include appropriate isotype controls and Fc receptor blocking reagents to minimize nonspecific binding.
• CD2 is expressed on T cells and NK cells; verify expression in your cellular model by co-staining with other lineage markers.
• The immunogen corresponds to the extracellular domain (aa 25-209), so the antibody likely recognizes an extracellular epitope; cell surface staining does not require permeabilization.
• Store at 2-8°C protected from light; avoid freezing, as repeated freeze-thaw cycles may reduce fluorophore stability.
• For multi-color flow cytometry panels, check spectral overlap of with other fluorophores and apply proper compensation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop supply of IVD raw materials, including validated anti-CD2 antibodies for T-cell studies, alongside ancillary reagents and customized sourcing services. From monoclonal antibodies to bulk conjugates, we support every step of your diagnostic or research workflow with reliable and scalable solutions.