Anti-Human CD1c Monoclonal Antibody for Flow Cytometry - P29017

Core Product Specifications and Parameters

Biological Background: CD1c Function and Localization

• T-cell surface glycoprotein CD1c is a member of the CD1 family of antigen-presenting molecules, encoded by the CD1C gene in humans.
• CD1c presents lipid and glycolipid antigens, both self and non-self, to T-cell receptors on natural killer T-cells, playing a central role in adaptive immunity.
• Unlike classical MHC molecules, CD1 proteins are non-polymorphic and specialize in lipid antigen presentation rather than peptide presentation.
• CD1c localizes to the cell membrane, endosome membrane, and lysosome membrane, enabling lipid antigen loading and surface display.
• Expression is detected on cortical thymocytes, certain T-cell leukemias, and various other tissues, indicating a regulated but broad tissue distribution.
• Structurally, CD1c contains an immunoglobulin domain, a transmembrane helix, and a disulfide bond; it is a glycoprotein with a mature molecular weight of approximately 38 kDa.

Experimental Guidance and Technical Tips

• The recombinant immunogen spans the extracellular region (amino acids 19–298), suggesting the antibody recognizes native CD1c. Confirm specificity using isotype controls or antigen-blocking experiments in the relevant detection system.
• For flow cytometry, titrate the antibody to determine the optimal staining concentration and achieve a suitable signal-to-noise ratio.
• has an excitation peak around 594 nm and emission around 615 nm; use appropriate laser and filter configurations (e.g., 594 nm laser, 615/20 nm filter) and protect the conjugate from prolonged light exposure.
• Consider including a viability dye and Fc receptor blocking step to minimize nonspecific binding in live-cell flow cytometry assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies such as this anti-CD1c clone, and bulk ancillary reagents. Our sourcing network streamlines procurement for both common and rare immunological targets, covering cell-surface markers, lipid-antigen-presenting molecules, and other critical components needed for immunoassay and flow cytometry development.