Anti-Human CD172b (SIRP-beta-1) Monoclonal Antibody for Flow Cytometry - O00241

Core Product Specifications and Parameters

Biological Background: Signal-regulatory Protein Beta-1 (CD172b) Function and Localization

• CD172b, also known as Signal-regulatory protein beta-1 (SIRP-beta-1) and CD172 antigen-like family member B, is an immunoglobulin-like cell surface receptor encoded by the SIRPB1 gene.
• It functions in the negative regulation of receptor tyrosine kinase-coupled signaling processes and mediates recruitment of tyrosine kinase SYK. Related references: PMID:10604985
• CD172b triggers activation of myeloid cells when associated with TYROBP (DAP12), acting as an activating counterpart in the SIRP family. Related references: PMID:10604985
• The protein localizes to the cell membrane and is primarily expressed in monocytes and dendritic cells.
• It contains an extracellular region with immunoglobulin-like domains, a single transmembrane helix, and a short cytoplasmic tail, characteristic of the SIRP family.
• Keywords indicate the presence of disulfide bonds, glycosylation sites, and alternative splicing, with a reported molecular weight of approximately 43 kDa (UniProt: 43.2 kDa).
• As a member of the signal-regulatory protein family, CD172b exhibits a transmembrane helix and a signal peptide, with implications in immune cell signaling and proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 30-371), which likely generates antibodies recognizing native CD172b on cell surfaces, making it suitable for flow cytometry.
• For FC, titrate the antibody to determine the optimal staining concentration for your specific cell type.
• Since CD172b is expressed on monocytes and dendritic cells, validate the antibody in these cell types or appropriate human peripheral blood mononuclear cells.
• Consider isotype controls and Fc receptor blocking to minimize non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate diagnostic development. This anti-CD172b antibody, targeting a key receptor in myeloid cell signaling, exemplifies our commitment to delivering high-quality immunoreagents for flow cytometry and immune monitoring. Explore our full range of antibodies and custom sourcing services to support your research and clinical applications.