Anti-Human CD166/ALCAM Monoclonal Antibody for Flow Cytometry - Q13740

Core Product Specifications and Parameters

Biological Background: CD166/ALCAM Function and Localization

• CD166/ALCAM is a cell adhesion molecule of the immunoglobulin superfamily, also known as activated leukocyte cell adhesion molecule (ALCAM) and MEMD, encoded by the ALCAM gene on human chromosome 3.
• It mediates both heterotypic (with CD6) and homotypic cell-cell contacts, promoting T-cell activation and proliferation, and contributing to the formation and maturation of the immunological synapse. Related references: PMID:15048703 PMID:16352806 PMID:24945728 PMID:15294938
• Through homotypic interactions, ALCAM inhibits endothelial cell migration and promotes tube formation, and mediates dendritic cell attachment to endothelial cells. Related references: PMID:15496415 PMID:23169771
• It is required for normal hematopoietic stem cell engraftment in the bone marrow and for maintaining normal numbers of hematopoietic stem cells. Related references: PMID:24740813
• Acts as a ligand for the LILRB4 receptor, thereby enhancing LILRB4-mediated inhibition of T cell proliferation. Related references: PMID:29263213
• An alternative splicing isoform functions as a decoy receptor, competing for the same interaction partners and inhibiting the activities of the membrane-bound isoforms; it promotes endothelial cell migration and inhibits tube formation.
• The protein localizes to the cell membrane and cell projections (axons and dendrites); a secreted isoform has also been identified.
• Detected in hematopoietic stem cells, lymph vessel endothelial cells, activated T and B cells, monocytes, thymic epithelial cells, neurons in the brain, and certain tumor cell lines (e.g., highly metastasizing melanoma). Related references: PMID:24740813 PMID:23169771 PMID:9502422 PMID:7760007
• Keywords: 3D-structure, Adaptive immunity, Alternative splicing, Cell adhesion, Cell membrane, Cell projection, Disulfide bond, Glycoprotein, Immunity, Immunoglobulin domain, Membrane, Proteomics identification, Reference proteome, Repeat, Secreted, Signal, Transmembrane, Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 28–526) of human CD166/ALCAM, making this conjugate suitable for detecting surface expression by flow cytometry.
• As a directly conjugated primary antibody (), no secondary reagent is required; protect from light during handling and staining to maintain fluorophore performance.
• For optimal flow cytometry results, titrate the antibody to determine the best concentration for your specific cell type and experimental conditions.
• Include appropriate isotype controls and fluorescence minus one (FMO) controls to distinguish specific binding.
• While validated for FC, the antibody may also be suitable for other applications like immunofluorescence or IHC, provided thorough validation in the relevant sample system is performed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive suite of IVD raw materials, supporting diagnostic manufacturers and research labs with validated monoclonal antibody conjugates for flow cytometry and other immunoassays. Through our integrated supply chain, we also provide bulk ancillary reagents, matched antibody pairs, and custom rare-target material sourcing, helping streamline your development from concept to clinical translation. For scientists studying immunology and cell adhesion, this anti-CD166/ALCAM conjugate exemplifies our commitment to delivering reliable, ready-to-use reagents coupled with expert technical support.