Anti-Human CD166/ALCAM Monoclonal Antibody for Flow Cytometry - Q13740

Core Product Specifications and Parameters

Biological Background: CD166/ALCAM Function and Localization

• Mediates heterotypic cell-cell adhesion via binding to CD6, contributing to T-cell activation and immunological synapse formation.
  Related references: PMID:15048703, PMID:16352806, PMID:24945728, PMID:15294938
• Mediates homotypic interactions between ALCAM-expressing cells, promoting endothelial tube formation and inhibiting endothelial migration.
  Related references: PMID:15496415, PMID:16352806, PMID:23169771
• Acts as a ligand for LILRB4, enhancing inhibition of T-cell proliferation.
  Related references: PMID:29263213
• Required for normal hematopoietic stem cell engraftment in bone marrow and maintenance of stem cell numbers.
  Related references: PMID:24740813
• Detected on hematopoietic stem cells, lymph vessel endothelial cells, activated T-cells, B-cells, monocytes, and dendritic cells. Isoforms are expressed in various tissues including vein/artery endothelium, astrocytes, and keratinocytes.
  Related references: PMID:9502422, PMID:7760007, PMID:15496415, PMID:24740813, PMID:23169771, PMID:15048703, PMID:16352806
• Localized to the cell membrane, as well as to axon and dendrite projections; a secreted isoform is also produced.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 28–526, which corresponds to the extracellular domain of CD166/ALCAM. This design supports applications like flow cytometry where surface antigen recognition is required.
• For flow cytometry, consider titrating the antibody on relevant human cell lines (e.g., Jurkat, PBMCs) or primary cells known to express CD166. Include appropriate isotype controls and fluorescence minus one (FMO) controls to ensure specificity.
• Validate antibody performance in your specific experimental system, as staining efficiency may vary with cell type, fixation, and permeabilization methods.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner for IVD raw material sourcing, offering validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers focusing on CD166/ALCAM in immunology and oncology, we provide streamlined access to high-quality raw materials that support diagnostic assay development. From concept to clinic, CamelBio bridges your supply needs with reliable, research-grade reagents.