Anti-Human CD15/SSEA-1 Monoclonal Antibody for Flow Cytometry - P22083

Core Product Specifications and Parameters

Biological Background: CD15/SSEA-1 (FUT4) Function and Localization

• The protein encoded by FUT4 (gene ID 2526) is Alpha-(1,3)-fucosyltransferase 4, a glycosyltransferase responsible for the final fucosylation step that creates the Lewis X (CD15/SSEA-1) carbohydrate antigen on N‑ and O‑linked glycoproteins. Related references: PMID:1702034 PMID:1716630 PMID:29593094

• Future 4 transfers fucose from GDP‑β‑L‑fucose to the distal N‑acetylglucosamine of type‑2 lactosamine chains (Galβ1‑4GlcNAc), most efficiently on non‑sialylated acceptors, thereby generating the Lewis X epitope. Related references: PMID:1716630 PMID:29593094

• In certain cell types, FUT4 can also fucosylate an internal GlcNAc residue to produce the VIM‑2 antigen, a ligand for E‑selectin (SELE). Related references: PMID:11278338 PMID:1716630

• Together with FUT7, FUT4 contributes to the biosynthesis of selectin ligands (sialyl Lewis X and 6‑sulfo sialyl Lewis X) that mediate leukocyte rolling and homing to sites of inflammation.

• FUT4 is a transmembrane protein that resides in the Golgi stack membrane, where it encounters its glycoprotein substrates during transit through the secretory pathway.

• Expression has been detected in bone marrow‑derived mesenchymal stem cells, cord blood immature promyelocytes, and in peripheral blood myeloid and lymphoid populations.

• The protein contains alternative initiation sites and is itself N‑glycosylated; it belongs to the glycosyltransferase 10 family and contains a signal‑anchor sequence.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from human CD15/SSEA-1; consider that the antibody may recognize a linear epitope that mimics the native carbohydrate structure or a peptide backbone of the carrier glycoprotein.
• For flow cytometry, include appropriate isotype controls and verify reactivity on a CD15‑positive cell line (e.g., HL‑60) and a CD15‑negative line (e.g., K562).
• For immunophenotyping applications, titrate the antibody to determine the optimal concentration for your specific cell population and staining protocol.
• The predicted molecular weight of the target enzyme is ~59 kDa; in western blotting (if attempted) be aware that the antibody may detect either the glycosylated FUT4 protein or its glycoprotein products bearing CD15.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated raw‑material sourcing partner for IVD manufacturers and research labs, offering streamlined access to a broad portfolio of validated antibodies, recombinant proteins, and ancillary reagents. Complementing this CD15/SSEA‑1 monoclonal antibody, we can supply paired antibody panels, optimized polyclonal reagents, and bulk ancillary materials for flow‑cytometry‑based hematology workflows. With our one‑stop approach, we help you accelerate assay development—from concept to clinical application—by bridging the gap between rare‑target sourcing and reliable supply chains.