Anti-Human CD158a/KIR2DL1 Monoclonal Antibody () for Flow Cytometry - P43626

Core Product Specifications and Parameters

Biological Background: CD158a/KIR2DL1 Function and Localization

• Encodes a killer cell immunoglobulin-like receptor (KIR) belonging to the immunoglobulin superfamily.
• Functions as an inhibitory receptor on natural killer (NK) cells, recognizing specific HLA-C alleles (e.g., w4 and w6) to suppress cell-mediated lysis.
• Localized to the cell membrane as a single-pass type I membrane protein with an extracellular region containing immunoglobulin-like domains.
• Expressed primarily on NK cells, playing a critical role in immune surveillance and self-tolerance.
• Contains a signal peptide, two immunoglobulin-like domains, and a transmembrane helix, as supported by domain annotations.
• Undergoes post-translational modifications including glycosylation and disulfide bond formation.
• Exists in multiple isoforms due to alternative splicing, potentially contributing to functional diversity.
• The canonical isoform has a calculated molecular weight of approximately 38.6 kDa, consistent with the observed 38-41 kDa band on gels.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 22-245), making the antibody well-suited for detecting surface-expressed KIR2DL1 in flow cytometry.
• For FC staining, titrate the antibody to establish the optimal concentration for your specific NK cell population.
• Validate specificity using positive controls such as KIR2DL1-transfected cells or NK cell lines with known expression profiles.
• As a fluorophore-conjugated antibody (), confirm compatibility with your cytometer’s excitation/emission filters (similar to FITC/GFP).
• Though reactivity is confirmed for human samples, cross-reactivity with other species is unlikely and should be verified if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw material sourcing, offering high-quality monoclonal antibodies like this anti-KIR2DL1 clone for NK cell and immune checkpoint research. From validated antibody pairs and optimized recombinant proteins to bulk ancillary reagents, we streamline procurement to accelerate your diagnostic development. Leverage our expertise to secure tailored raw materials and technical support from concept to clinic.