Anti-Human CD158 (KIR2DL1/S1/S2/S3/S5) Monoclonal Antibody for Flow Cytometry - Q14954 / Q14953 / Q14952 / P43631 / P43626

Core Product Specifications and Parameters

Biological Background: CD158 (KIR2DL1/S1/S2/S3/S5) Function and Localization

• The CD158 cluster encompasses a family of polymorphic killer-cell immunoglobulin-like receptors (KIRs), including KIR2DL1, KIR2DS1, KIR2DS2, KIR2DS3, and KIR2DS5, which play critical roles in regulating NK cell activity.
• KIR2DS1 (CD158H) acts as an activating receptor on NK cells, recognizing specific HLA-C alleles such as w6 and promoting NK cell activation rather than inhibition.
• These receptors are single-pass type I membrane proteins localized at the cell surface, with extracellular immunoglobulin-like domains and cytoplasmic signaling motifs.
• The extracellular region contains two Ig-like domains, involved in ligand binding, while the transmembrane and cytoplasmic domains mediate signal transduction.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, essential for proper folding and expression on the cell membrane.
• NK cell expression of these receptors is clonally distributed, contributing to the diversity of NK cell responses and self-tolerance mechanisms.
• Keywords associated with KIR2DS1 include: cell membrane, disulfide bond, glycoprotein, immunoglobulin domain, membrane, receptor, reference proteome, repeat, signal, transmembrane, transmembrane helix.

Experimental Guidance and Technical Tips

• This antibody, raised against the extracellular domain of human CD158, is suitable for flow cytometric detection of surface KIR expression on NK cells.
• Optimize antibody titration for your specific cell type and experimental conditions to achieve optimal staining resolution.
• Consider using appropriate isotype controls and Fc receptor blocking to minimize non-specific background.
• In multicolor flow cytometry panels, verify fluorophore compatibility and potential spectral spillover with other conjugated antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this anti-CD158 clone. Whether you require bulk quantities, ancillary reagents, or rare target sourcing, we streamline procurement from concept to clinic. Explore our T/NK cell marker antibodies to accelerate your immunophenotyping assay development.