Anti-Human CD153 Monoclonal Antibody for Flow Cytometry - P32971

Core Product Specifications and Parameters

Biological Background: CD153 (CD30L) Function and Localization

• Tumor necrosis factor ligand superfamily member 8 (TNFSF8), also known as CD153 or CD30 ligand (CD30-L), is a type II transmembrane protein belonging to the tumor necrosis factor (TNF) ligand family.
• It functions as a cytokine that specifically binds to the receptor TNFRSF8/CD30, triggering downstream signaling pathways that promote T-cell proliferation and immune responses.
• The protein is anchored to the cell membrane via an uncleaved signal-anchor sequence, with a predicted transmembrane helix domain; it may also be proteolytically processed to generate a soluble form.
• Post-translational modifications include glycosylation and the presence of a disulfide bond, which are critical for its structural stability and receptor binding.
• UniProt annotates CD153 with keywords such as cytokine, transmembrane, glycoprotein, and disulfide bond, reflecting its functional and structural features.

Experimental Guidance and Technical Tips

• This -conjugated monoclonal antibody is ready-to-use for direct flow cytometry without requiring a secondary detection step, minimizing non-specific binding.
• The immunogen encompasses the extracellular domain of CD153 (amino acids 63-234), which corresponds to the region involved in receptor interaction; it is expected to recognize both membrane-bound and shed forms of the protein.
• For flow cytometry, optimal staining concentrations and conditions should be empirically determined using appropriate positive and negative cell lines expressing CD153.
• Include an appropriate isotype control and live/dead cell discrimination in your flow cytometry panel to ensure specific and accurate detection.
• Although validated for flow cytometry, other applications (e.g., immunofluorescence) may be explored with careful optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of high-quality IVD raw materials for diagnostic developers and researchers. This anti-human CD153 monoclonal antibody supports investigations into CD30/CD153-mediated immune signaling and T-cell biology. As your integrated sourcing partner, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and expertise in rare-target raw-material supply to accelerate your assay development from concept to clinic.