Anti-Human CD150/SLAM Monoclonal Antibody () for FC - Q13291

Core Product Specifications and Parameters

Biological Background: CD150/SLAM Function and Localization

• SLAMF1 encodes the signaling lymphocytic activation molecule (SLAM), also known as CD150, CDw150, or IPO-3, a member of the SLAM family of immunoglobulin-like receptors.
• SLAM acts as a self-ligand receptor that modulates the activation and differentiation of immune cells, playing a critical role in both innate and adaptive immunity.
• SLAMF1 signal transduction operates through two distinct modes: one dependent on the adaptor SH2D1A/SAP, which recruits FYN kinase to phosphorylate and activate SLAMF1, and another involving PTPN11/SHP-2 phosphatase. Related references: PMID:11806999 PMID:12458214
• In B-cells, SLAMF1 activates the ERK and Akt signaling pathways via SYK and SH2D1A, and also stimulates p38 MAPK and JNK1/2 pathways, thereby influencing antibody responses. Related references: PMID:20231852
• In macrophages, SLAMF1 participates in the innate immune response against Gram-negative bacteria by recognizing bacterial porins OmpC/OmpF, promoting phagosome maturation and PI3K complex II recruitment for reactive oxygen species production. Related references: PMID:20818396
• SLAMF1 serves as a cellular receptor for measles virus, including isoform 4, facilitating viral entry and infection.
• Constitutively expressed on peripheral blood memory T-cells, immature thymocytes, and a subset of B-cells, SLAMF1 is rapidly induced on naïve T-cells upon activation. Isoform 4 shows elevated expression in various central nervous system tumors. Related references: PMID:7617038 PMID:9091591 PMID:25710480
• The protein localizes predominantly to the cell membrane and can be secreted; it is post-translationally modified by glycosylation, phosphorylation, and disulfide bond formation.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal extracellular domain (amino acids 21-237), which encompasses the ligand-binding region; this design likely preserves native conformational epitopes suitable for flow cytometry.
• For flow cytometry applications, titrate the antibody to determine the optimal staining concentration and include appropriate controls such as isotype controls and unstimulated vs. activated cell samples.
• As a direct conjugate, this antibody enables single-step staining, minimizing background and simplifying multicolor panel design.
• CD150 is expressed on activated T-cells, memory B-cells, dendritic cells, and thymocytes; validate target expression in your specific cell type using a positive control (e.g., PHA-stimulated PBMCs).
• The antibody is validated for human samples; cross-reactivity with other species has not been tested, so confirm species specificity if used in animal models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop platform for IVD raw materials, supporting diagnostic manufacturers from research to clinical application. For immune profiling and infectious disease studies, we offer validated antibodies like this anti-CD150/SLAM clone, along with recombinant proteins, antibody pairs, and bulk ancillary reagents. Our global sourcing network and technical expertise help accelerate your assay development with reliable, customized solutions.