Anti-Human CD146 Monoclonal Antibody () for Flow Cytometry - P43121

Core Product Specifications and Parameters

Biological Background: CD146 Function and Localization

• CD146, also known as MUC18, MCAM, HEMCAM, METCAM, or MelCAM, is a cell surface glycoprotein belonging to the immunoglobulin superfamily and encoded by the MCAM gene.
• It functions as a calcium-independent cell adhesion molecule, playing a critical role in maintaining the cohesion of the endothelial monolayer at intercellular junctions in vascular tissue.
• CD146 expression in melanoma cells is associated with enhanced interaction with vascular elements, potentially facilitating hematogenous tumor spread and metastasis.
• It also acts as a surface receptor that triggers intracellular signaling, including tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in intracellular calcium concentration.
• During embryonic development, CD146 appears on the surface of neural crest cells, suggesting an adhesion role in cell migration.
• In normal adult tissues, CD146 expression is largely restricted to vascular smooth muscle and endothelial cells; its upregulation strongly correlates with tumor progression and metastasis in malignant melanoma.
• Post‑translational modifications include glycosylation and phosphorylation; the protein contains a signal sequence, a transmembrane helix, and multiple immunoglobulin‑like domains with disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 24–559, corresponding to the extracellular domain of CD146, making this antibody suitable for detecting surface‑expressed CD146 in non‑permeabilized cells by flow cytometry.
• Optimal staining conditions (antibody concentration, incubation time, and temperature) should be titrated by the end user in the relevant cell system (e.g., human endothelial or melanoma cell lines).
• Protect the conjugate from light during staining and storage to preserve the fluorophore.
• Include appropriate isotype controls and live/dead discrimination to ensure specific detection.
• The antibody may also be applicable in other immunoassays that detect native extracellular epitopes, but these applications require independent validation.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw‑material partner, CamelBio streamlines procurement for diagnostic developers and researchers working on CD146‑related oncology or vascular biology projects. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your assay development. Let CamelBio’s sourcing expertise be your bridge from concept to clinic.