Anti-Human CD141/Thrombomodulin Monoclonal Antibody for Flow Cytometry - P07204

Core Product Specifications and Parameters

Biological Background: Thrombomodulin (CD141) Function and Localization

• Thrombomodulin (also known as fetomodulin, CD141, TM, THRM) is an endothelial cell receptor critical for hemostasis, coagulation, fibrinolysis, inflammation, and angiogenesis. Related references: PMID:10761923
• It acts as a cofactor for thrombin-mediated activation of protein C (PROC), initiating the anticoagulant pathway on vascular endothelial surfaces. Related references: PMID:29323190, PMID:33836597, PMID:9395524
• Additionally, thrombomodulin enhances activation of carboxypeptidase B2 (CPB2), which inhibits fibrinolysis by cleaving C-terminal basic amino acids from kinins and anaphylatoxins. Related references: PMID:26663133
• It also suppresses inflammation independently of its anticoagulant activity by binding to and sequestering HMGB1, preventing its interaction with receptors like RAGE. Related references: PMID:15841214
• Subcellularly, thrombomodulin is a single-pass type I membrane protein located on the plasma membrane of endothelial cells.
• Tissue expression is highly specific: only endothelial cells synthesize thrombomodulin.
• The protein contains structural features such as an EGF-like domain, disulfide bonds, and is glycosylated; it has a molecular weight of ~60 kDa (UniProt calculates ~60.3 kDa).
• Clinically, mutations in THBD are associated with thrombophilia and hemolytic uremic syndrome, underscoring its importance in vascular homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 19-515, encompassing the entire extracellular domain; therefore, the antibody is expected to recognize the native conformation of thrombomodulin on live endothelial cells.
• This antibody is directly conjugated to , a far-red fluorophore suitable for flow cytometry without the need for secondary staining. consider titrating the antibody to optimize the signal-to-noise ratio in your specific experimental system.
• Validate staining in a relevant cell type known to express thrombomodulin, such as primary endothelial cells or endothelial cell lines; isotype controls are essential for accurate gating.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, technical services, and consulting, supporting every stage from concept to clinic. This anti-human CD141/Thrombomodulin antibody exemplifies our offering for researchers in coagulation, inflammation, and vascular biology. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing raw materials for rare or specialized targets to accelerate your assay development and manufacturing.