Anti-Human CD140b/PDGFRβ Monoclonal Antibody for Flow Cytometry - P09619

Core Product Specifications and Parameters

Biological Background: PDGFRβ Function and Localization

• PDGFRβ is a receptor tyrosine kinase that binds PDGFB and PDGFD homodimers, as well as PDGFA-PDGFB heterodimers, regulating cell proliferation, survival, migration, and differentiation.
• It plays essential roles in embryonic development, particularly in cardiovascular system formation, angiogenesis, and pericyte recruitment to endothelial cells.
• PDGFRβ signaling is crucial for vascular smooth muscle cell migration and neointima formation at vascular injury sites.
• The receptor is also required for normal kidney glomerulus development, mediating mesangial cell recruitment and capillary network branching.
• Ligand binding activates multiple intracellular pathways including PI3K-AKT, RAS-MAPK, and PLCγ, leading to cytoskeletal rearrangements and membrane ruffling.
• PDGFRβ localizes primarily to the cell membrane, but also resides in cytoplasmic vesicles and the lysosome lumen after internalization.
• Post-translational modifications include glycosylation, phosphorylation, disulfide bond formation, and ubiquitin conjugation, which regulate receptor activity and turnover.

Experimental Guidance and Technical Tips

• This antibody was raised against the extracellular domain (aa 33–530) of human PDGFRβ; it is expected to recognize the native conformation on live cells, but titration and validation in the relevant cell system are advised.
• For flow cytometry, use an appropriate rabbit monoclonal isotype control conjugated to the same fluorophore, and include Fc receptor blocking when working with cells expressing high FcR levels.
• PDGFRβ is expressed on vascular smooth muscle cells, pericytes, fibroblasts, and some tumor cell lines; verify expression in your model prior to staining.
• When co-staining with other antibodies, ensure fluorophore compatibility and perform appropriate compensation controls.
• Fixation with paraformaldehyde may affect epitope accessibility; if fixation is required, test both live and fixed conditions to optimize staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop platform for IVD raw materials, including this high-affinity anti-PDGFRβ monoclonal antibody. Our portfolio covers validated antibody pairs, bulk ancillary reagents, and access to rare-target raw materials, streamlining procurement for diagnostic manufacturers and research laboratories worldwide. With end-to-end support from development to scale-up, CamelBio accelerates your projects from concept to clinic.