Anti-Human CD138/Syndecan-1 Rabbit Monoclonal Antibody for Flow Cytometry - P18827

Core Product Specifications and Parameters

Biological Background: Syndecan-1 Function and Localization

• Syndecan-1 (CD138, SDC1) is a cell surface proteoglycan that contains both heparan sulfate and chondroitin sulfate chains, linking the cytoskeleton to the interstitial matrix.
• It regulates exosome biogenesis in concert with SDCBP and PDCD6IP, influencing extracellular vesicle formation. Related references: PMID:22660413
• Able to induce its own expression in dental mesenchymal and epithelial cells via an MSX1-mediated pathway (by similarity).
• Subcellularly localized to the membrane, secreted, and found in extracellular exosomes, reflecting its dynamic trafficking.
• Detected in placenta and fibroblasts at the protein level, indicating tissue-specific expression patterns. Related references: PMID:32337544 PMID:36213313
• Classified as a glycoprotein, phosphoprotein, and proteoglycan; its heparan sulfate modifications are critical for ligand binding and signaling (by similarity).

Experimental Guidance and Technical Tips

• This -conjugated antibody is designed for direct flow cytometry detection of cell surface Syndecan-1 on human cells.
• Use a titration series to determine the optimal antibody concentration for your specific cell type and experimental conditions.
• Keep in mind that Syndecan-1 can be shed from the cell surface, which may reduce staining intensity in certain culture conditions; consider analyzing both surface and total expression if needed.
• For best results, validate the staining protocol using a relevant positive control (e.g., cells known to express high levels of Syndecan-1, such as plasma cells) and an isotype control antibody conjugate.
• The immunogen corresponds to the extracellular domain (aa 23-251), ensuring recognition of the native extracellular epitope; avoid over-fixation that might alter epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your diagnostic and research programs with one-stop access to premium IVD raw materials, including validated conjugated antibodies like this anti-CD138 (Syndecan-1) mAb for flow cytometry. Beyond individual reagents, we offer integrated sourcing solutions—matched antibody pairs, bulk ancillary reagents, and custom procurement of rare-target antibodies—to accelerate your assay development from concept to clinic. Our expertise in exosome biology and cell surface proteoglycans helps ensure you have the right tools for robust, reproducible results.