Anti-Human CD138/Syndecan-1 Monoclonal Antibody (Conjugate) for Flow Cytometry - P18827

Core Product Specifications and Parameters

Biological Background: Syndecan-1 Function and Localization

• Syndecan-1 (also known as CD138, SDC1, SYND1) is a transmembrane heparan sulfate proteoglycan that belongs to the syndecan family.
• It links the cytoskeleton to the interstitial matrix through its heparan sulfate and chondroitin sulfate glycosaminoglycan chains (By similarity).
• Syndecan-1 regulates exosome biogenesis in concert with SDCBP and PDCD6IP. Related references: PMID:22660413
• It can induce its own expression in dental mesenchymal and neighboring epithelial cells via an MSX1-mediated pathway (By similarity).
• Subcellularly, Syndecan-1 localizes to the plasma membrane, is secreted, and is found in extracellular exosomes.
• Tissue expression has been detected in placenta and in fibroblasts at the protein level. Related references: PMID:32337544, PMID:36213313
• Post-translational modifications include glycosylation (heparan sulfate and chondroitin sulfate attachment), phosphorylation, and proteolytic shedding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 23–251) of human CD138/Syndecan-1, suggesting the antibody recognizes the exposed ectodomain on the cell surface.
• Directly conjugated to , this antibody is validated for flow cytometry (FC); consider optimizing antibody titration and including appropriate isotype and FMO controls.
• The core protein mass is approximately 32 kDa, but extensive glycosylation often results in a higher apparent molecular weight in denaturing gels.
• Antigen retrieval or fixation protocols should be tested to ensure epitope preservation, as harsh conditions may alter the extracellular domain conformation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted provider for diagnostics manufacturers and research laboratories, offering comprehensive access to IVD raw materials, technical services, and consulting from concept to clinic. For your CD138/Syndecan-1 research and assay development needs, we supply validated monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing upon request. Partner with CamelBio to streamline your diagnostic workflow with reliable, high-quality raw materials.