Anti-Human CD124/IL-4Rα Monoclonal Antibody for Flow Cytometry - P24394

Core Product Specifications and Parameters

Biological Background: CD124/IL-4Rα Function and Localization

• IL4R encodes the interleukin-4 receptor subunit alpha, which functions as a receptor for both IL-4 and IL-13, coupling to the JAK1/2/3-STAT6 signaling pathway (PubMed:17030238). Related references: PMID:17030238
• This receptor is pivotal in promoting T-helper 2 (Th2) cell differentiation and mediating IgE production, as well as chemokine and mucus secretion during allergic inflammation.
• In certain cell types, IL4R can also signal through insulin receptor substrates (IRS1/IRS2), linking it to metabolic pathways.
• Alternative splicing produces a soluble form (sIL4R) that acts as a decoy receptor, inhibiting IL-4-mediated cell proliferation and IL-5 up-regulation by T-cells.
• IL4R is localized at the cell membrane and can be secreted; it is highly expressed in activated T-cells (isoforms 1 and 2).
• The protein undergoes post-translational modifications including glycosylation and phosphorylation, and contains disulfide bonds, characteristic of the immunoglobulin superfamily.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 24-232), which likely ensures recognition of the native receptor; consider verifying epitope accessibility in your model.
• The conjugate enables direct detection without secondary antibodies, reducing background; validate in the relevant sample system (e.g., activated T-cells) with appropriate isotype controls.
• If quantifying surface expression, wash cells thoroughly to remove any secreted sIL4R that could compete with binding.

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