Anti-Human CD124/IL-4Rα Monoclonal Antibody for FC - P24394

Core Product Specifications and Parameters

Biological Background: CD124/IL-4Rα Function and Localization

• CD124 (IL-4Rα) is the high-affinity receptor subunit for interleukin-4 and a component of the interleukin-13 receptor, with multiple isoforms including a soluble form (sIL4R) that acts as a decoy receptor (PubMed:17030238). Related references: PMID:17030238
• Ligand binding activates the JAK1/2/3-STAT6 signaling cascade, promoting Th2 lymphocyte differentiation and regulating immune responses.
• IL-4Rα signaling drives IgE class switching, and chemokine and mucus production, key events in allergic inflammation.
• In certain cell types, IL-4Rα can recruit insulin receptor substrates IRS1/IRS2, linking to metabolic signaling.
• Soluble IL-4R (sIL4R) naturally antagonizes IL-4 by blocking IL-4-mediated cell proliferation and IL-5 up-regulation in T-cells.
• The receptor is localized at the cell membrane and can be secreted; it is abundantly expressed on activated T-cells.
• The protein undergoes co- and post-translational modifications, including N-glycosylation, disulfide bond formation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the ligand-binding extracellular region (aa 24-232) of human CD124, making the antibody suitable for detecting surface receptor expression in live cells by flow cytometry.
• As a fluorophore-conjugated () monoclonal antibody, it allows direct one-step staining; titrate to determine the optimal concentration for your assay.
• Use an isotype control and unstimulated cells as negative controls; for assessing IL-4-induced responses, consider treating cells with recombinant IL-4 before staining.
• The antibody has been tested for human reactivity; if applying to non-human samples, validate cross-reactivity experimentally.
• While intended for flow cytometry, other applications may be possible; thorough in-house validation is essential if you plan to use it in Western blot or ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers in the immunology and allergy field with a comprehensive portfolio of IVD raw materials. In addition to this anti-CD124/IL-4Rα monoclonal antibody, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your assay development from concept to clinic. Explore our catalog for reliable, high-quality reagents tailored for flow cytometry and immunoassay applications.