Anti-Human CD117/c-Kit Monoclonal Antibody for Flow Cytometry - P10721

Core Product Specifications and Parameters

Biological Background: CD117/c-Kit Function and Localization

• CD117/c-Kit is a type III receptor tyrosine kinase that serves as the cell-surface receptor for stem cell factor (KITLG/SCF); it is encoded by the KIT proto-oncogene and also known as mast/stem cell growth factor receptor, piebald trait protein (PBT), and p145 c-kit.
• Upon ligand binding, KIT activates multiple downstream signaling cascades including PI3K/AKT, RAS/RAF/MAPK, and STAT pathways, regulating cell survival, proliferation, and differentiation in hematopoietic, germ cell, and melanocyte lineages.
• The protein contains an extracellular region with five immunoglobulin-like domains, a single transmembrane helix, and an intracellular tyrosine kinase domain, with documented alternative splicing producing isoforms.
• KIT is critical for mast cell development and function; it is also essential for gametogenesis, with expression detected in testicular spermatogonia, interstitial Leydig cells, and ejaculated spermatozoa. Related references: PMID:20601678
• Subcellularly, KIT is primarily located at the cell membrane but can also be detected in the cytoplasm upon activation or internalization.
• Post-translational modifications include glycosylation, disulfide bond formation, and ubiquitination, which regulate its stability and signaling.
• The protein has a molecular weight of approximately 109-110 kDa and is classified under tyrosine-protein kinase receptors with ATP-binding and metal-binding properties.
• Clinically, mutations in KIT are associated with piebaldism, mastocytosis, gastrointestinal stromal tumors, and other malignancies, making it a diagnostic and therapeutic target.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 26-520, covering the entire extracellular domain of human CD117/c-Kit. This design may enhance the likelihood of recognizing native, cell-surface receptor on live cells, making it suitable for flow cytometry applications.
• When using this -conjugated antibody for flow cytometry, consider titrating the antibody to determine the optimal staining concentration for your specific cell type and experimental conditions.
• Include appropriate isotype controls and viability dyes to ensure accurate population gating, especially when analyzing rare hematopoietic stem cells or mast cells.
• Validate the antibody performance in your sample matrix (e.g., peripheral blood, bone marrow, cell lines) and consider using known positive and negative controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering everything from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. With this anti-CD117/c-Kit reagent, we support research in hematopoiesis, oncology, and stem cell biology, and we can also assist with sourcing rare-target raw materials for your diagnostic development needs.