Anti-Human CD116 Monoclonal Antibody for Flow Cytometry - P15509

Core Product Specifications and Parameters

Biological Background: CD116 Function and Localization

• CD116, the granulocyte-macrophage colony-stimulating factor receptor subunit alpha (CSF2RA, also known as CDw116), is a low-affinity receptor for GM-CSF that mediates hematopoietic cell proliferation, differentiation, and activation.
• It is a single-pass type I membrane protein that transduces ligand-induced signals through association with the common beta chain (CSF2RB) to form the high-affinity receptor complex.
• The receptor localizes primarily to the cell membrane, with soluble isoforms generated by alternative splicing and proteolytic shedding; it is also annotated as secreted.
• Multiple protein isoforms arise from alternative splicing and differential glycosylation, contributing to the observed molecular weight range of 26 kDa to 49 kDa.
• CD116 contains three extracellular fibronectin type III domains, a conserved WSXWS motif, and disulfide bonds that stabilize its structure, as supported by the 3D-structure keyword.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, which are critical for proper folding and ligand binding.
• Genetic variants in CSF2RA are associated with disease phenotypes, including pulmonary alveolar proteinosis, as indicated by the disease variant keyword.

Experimental Guidance and Technical Tips

• The immunogen encompassing amino acids 23–320 of the extracellular domain ensures recognition of native CD116 on the cell surface, making the antibody suitable for flow cytometry without permeabilization.
• Titrate the antibody to determine the optimal staining concentration for your specific cell type, as CD116 expression varies among hematopoietic lineages.
• Include appropriate controls: isotype control, fluorescence-minus-one, and cell lines with known CD116 expression (e.g., TF-1 or U937) to validate specificity.
• Consider that glycosylation differences among isoforms may affect epitope accessibility or fluorescence intensity; verify performance in the relevant sample matrix.
• If adapting for other applications, re-validate thoroughly, as the conjugate may influence biochemical binding properties compared to unconjugated antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CD116 antibody supports research into GM-CSF signaling and hematopoiesis, and CamelBio’s expansive portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development.