Anti-Human B7-H2/CD275 Monoclonal Antibody for FC - O75144

Core Product Specifications and Parameters

Biological Background: B7-H2/CD275 Function and Localization

• B7-H2/CD275 (ICOSLG) is a single-pass type I membrane protein belonging to the immunoglobulin superfamily; also known as B7 homolog 2, B7RP-1, or GL50 (UniProt O75144).
• Serves as the ligand for the T-cell-specific receptor ICOS, delivering a critical costimulatory signal that drives T-cell proliferation and cytokine secretion (PubMed:11007762, PubMed:11023515, PubMed:30498080). Related references: PMID:11007762, PMID:11023515, PMID:30498080
• Also promotes B-cell proliferation and differentiation into plasma cells, and is implicated in local tissue responses during inflammation and the co‑stimulation of memory T-cell functions.
• In endothelial cells, ICOSLG is required for efficient neutrophil transmigration in response to chemoattractants such as CXCL8/IL8 or fMLP (PubMed:30498080). Related references: PMID:30498080
• Predominantly localized to the cell membrane; expressed on peripheral blood B‑cells, monocytes, monocyte-derived dendritic cells, and is widely detected across lymphoid and non‑lymphoid tissues.
• Features immunoglobulin-like domains, disulfide bonds, multiple glycosylation sites, and an alternative splicing mechanism, underscoring its roles in adaptive immunity and B‑cell activation (Keywords: 3D-structure, Adaptive immunity, Alternative splicing, B-cell activation, Disulfide bond, Glycoprotein).

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 19‑256, covering the extracellular domain responsible for ICOS binding; this design targets the functionally relevant region.
• For flow cytometry (FC), titrate the antibody to determine the optimal staining concentration on human lymphocytes or monocyte‑derived dendritic cells; include appropriate isotype controls.
• Consider validating expression in the specific leukocyte subset of interest, as ICOSLG levels can vary with activation status and tissue type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive range of IVD raw materials, from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents. For immune‑oncology and inflammation programs, we offer reliable sourcing of B7‑H2/CD275 reagents, including rare‑target antibodies, along with technical consulting to help accelerate your assay development pipeline.