Anti-Human Albumin Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P02768

Core Product Specifications and Parameters

Biological Background: Albumin Function and Localization

• Albumin (ALB) is the predominant plasma protein, secreted from the liver, and constitutes about 50% of blood serum protein.
• Its primary physiological role is to maintain colloidal osmotic pressure and transport fatty acids, hormones, bilirubin, and various drugs.
• Albumin is a major zinc transporter in plasma, binding about 80% of all plasma zinc (PubMed:19021548). Related references: PMID:19021548
• It also binds approximately 45% of circulating calcium and magnesium, with affinity order zinc > calcium > magnesium.
• Albumin binds the bacterial siderophore enterobactin and inhibits enterobactin‑mediated iron uptake by E. coli, thereby limiting bacterial iron utilization and growth (PubMed:6234017). Related references: PMID:6234017
• The mature protein has a molecular weight of 69 kDa and features multiple disulfide bonds and three homologous domains.
• Albumin undergoes extensive post‑translational modifications, including glycation, phosphorylation, and methylation, and is subject to proteolytic cleavage.
• It is localized extracellularly, predominantly in plasma.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length secreted form (amino acids 25‑609), suggesting this polyclonal antibody likely recognizes both native and denatured albumin.
• For Western blot, expect a band around 69 kDa; reducing conditions may be used.
• For IHC-P, consider antigen retrieval and validate staining in liver or plasma‑rich tissues.
• For IF/ICC, note that albumin is secreted; intracellular staining may reflect re‑uptake or synthesis in hepatocytes.
• For ELISA, the antibody can be considered as a capture or detection reagent; optimize pairing for your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials for albumin and other plasma protein targets. We provide validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. Through our global network, we help you source rare‑target raw materials efficiently, ensuring a seamless path from concept to clinic.