Anti-Human AGTR2 Monoclonal Antibody for Flow Cytometry - P50052

Core Product Specifications and Parameters

Biological Background: AGTR2 Function and Localization

• The AGTR2 gene encodes the type-2 angiotensin II receptor (AT2 receptor), a G protein-coupled receptor that mediates the effects of the vasoconstricting peptide angiotensin II, involved in blood pressure regulation and cardiovascular function. Related references: PMID:28379944, PMID:29967536, PMID:31899086
• Primarily signals through non-canonical G protein- and beta-arrestin independent pathways, leading to modulation of cell proliferation and apoptosis.
• Cooperates with the tumor suppressor MTUS1 to inhibit ERK2 activation and cell proliferation. Related references: PMID:15123706
• Subcellular localization: Predominantly at the cell membrane, with possible internalization upon ligand binding.
• Tissue specificity: In adult, highly expressed in myometrium; lower levels in adrenal gland and fallopian tube; also expressed in cerebellum. Very highly expressed in fetal kidney and intestine.
• Post-translational modifications include disulfide bond formation and N-linked glycosylation, essential for receptor folding and function.
• Belongs to the G protein-coupled receptor superfamily, characterized by seven transmembrane helices and involved in diverse signaling processes.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal extracellular region (amino acids 1-45) of human AGTR2; this sequence may be critical for recognizing the native receptor conformation on live cells, making it well-suited for flow cytometry.
• As a directly -conjugated antibody, secondary detection reagents are not required, potentially reducing background and assay time. Titrate the antibody to determine the optimal concentration for each cell type and experimental condition.
• For flow cytometry, include appropriate isotype controls and viability dyes to accurately gate live AGTR2-positive cell populations.
• Validate antibody specificity using AGTR2-overexpressing cell lines or by parallel staining of known positive (e.g., myometrium-derived cells) and negative human samples.
• AGTR2 expression can be modulated by hormonal or pathological states; consider treating cells with angiotensin II or other regulators when functional studies are intended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research institutes, and clinical laboratories with one-stop access to high-quality IVD raw materials, covering the entire development pipeline from concept to clinic. This anti-AGTR2 antibody supports research in cardiovascular diseases, hypertension, and receptor pharmacology. Beyond this product, CamelBio supplies validated antibody pairs, recombinant proteins, bulk ancillary reagents, and offers custom sourcing for rare-target raw materials to accelerate your diagnostic assay development.