Anti-HTRA2 Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - O43464

Core Product Specifications and Parameters

Biological Background: HTRA2 Function and Localization

• HTRA2 (High temperature requirement protein A2), also known as Omi stress-regulated endoprotease or serine protease 25, is a mitochondrial serine protease encoded by the HTRA2 gene.
• It promotes apoptosis through multiple mechanisms: relieving inhibition of BIRC proteins (such as BIRC6) on caspases (CASP3, CASP7, CASP9), leading to increased caspase activity; or via a caspase-independent, serine protease-dependent pathway. Additionally, HTRA2 cleaves THAP5 to promote its degradation during apoptosis. (PubMed:10873535, 15200957, 36758104, 36758105, 19502560) Related references: PMID:10873535, PMID:15200957, PMID:36758104, PMID:36758105, PMID:19502560
• Alternative splicing may generate a proteolytically inactive isoform of HTRA2.
• Subcellularly, HTRA2 localizes primarily to the mitochondrial intermembrane space and membrane, but is also found in the endoplasmic reticulum.
• It is ubiquitously expressed across tissues.
• HTRA2 is associated with neurodegenerative disorders, particularly Parkinson disease, and is listed under keywords such as apoptosis, serine protease, and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 334–458) of human HTRA2, which may contribute to good epitope accessibility and specificity.
• For Western blot (WB), a band of approximately 49 kDa is expected in lysates from human, mouse, and rat samples. Consider using protease inhibitors during sample preparation to avoid degradation.
• For immunohistochemistry paraffin sections (IHC-P), optimization of antigen retrieval (e.g., heat-induced epitope retrieval at pH 6 or 9) and antibody concentration is recommended. Use appropriate controls.
• For immunoprecipitation (IP), the polyclonal nature of the antibody may efficiently capture the full-length protein; validate capture and detection conditions empirically.
• For ELISA, consider pairing with a specific detection antibody and check cross-reactivity with related serum proteins if using complex samples.
• Cross-reactivity with mouse and rat orthologs makes this antibody suitable for rodent model studies; however, confirm cross-reactivity in your specific application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for diagnostic manufacturers and research laboratories, offering a comprehensive portfolio of IVD raw materials—including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For targets like HTRA2, central to apoptosis and neurodegeneration research, CamelBio streamlines the procurement of high-quality raw materials and provides technical support to bridge the gap from concept to clinic. Contact us to discuss your specific HTRA2 antibody sourcing needs or to explore custom assay development solutions.