Anti-HTRA2 Rabbit Monoclonal Antibody for WB, ELISA - O43464

Core Product Specifications and Parameters

Biological Background: HTRA2 Function and Localization

• HTRA2 is a mitochondrial serine protease that promotes apoptosis by cleaving and inactivating inhibitor of apoptosis proteins (IAPs) such as BIRC6, enhancing caspase activity (PubMed:10873535, PubMed:15200957, PubMed:36758104, PubMed:36758105). Related references: PMID:10873535 PMID:15200957 PMID:36758104 PMID:36758105
• It also triggers apoptosis via a caspase-independent mechanism that depends on its serine protease activity (PubMed:15200957). Related references: PMID:15200957
• The canonical isoform is catalytically active; however, alternative splicing generates an isoform that appears proteolytically inactive.
• HTRA2 resides primarily in the mitochondrial intermembrane space and can associate with the mitochondrial membrane; it is also detected in the endoplasmic reticulum.
• It is ubiquitously expressed in human tissues.
• Cleaves THAP5 and promotes its degradation during apoptosis (PubMed:19502560). Related references: PMID:19502560
• Keywords: Serine protease, Apoptosis, Neurodegeneration, Parkinson disease, Parkinsonism, Epilepsy, Alternative splicing, Ubl conjugation, Zymogen.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100-200 of human HTRA2; cross-reactivity with mouse and rat suggests sequence conservation in this region.
• For Western blot, a band of approximately 49 kDa is expected in samples from human, mouse, and rat.
• ELISA applications should be optimized; consider testing with recombinant HTRA2 or cell lysates.
• Due to HTRA2's role in apoptosis, expression levels may vary under different cellular conditions; include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a comprehensive sourcing solution for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. With expertise in rare target sourcing, we help streamline your assay development from concept to clinic. Explore our anti-HTRA2 rabbit monoclonal antibody to support your apoptosis and neurodegeneration studies.