Anti-HTR4 Rabbit Polyclonal Antibody for WB, ELISA - Q13639

Core Product Specifications and Parameters

Biological Background: HTR4 Function and Localization

• HTR4 encodes a G protein-coupled receptor for serotonin (5-hydroxytryptamine), which acts as a neurotransmitter, hormone, and mitogen. Related references: PMID:10821780 PMID:16102731 PMID:35714614

• Upon ligand binding, HTR4 couples to G(s) alpha proteins, activating adenylate cyclase and triggering downstream cAMP signaling. Related references: PMID:16102731 PMID:35714614

• The receptor is expressed in the ileum, brain, and cardiac atrium, with isoform-specific distributions across cardiovascular tissues.

• HTR4 localizes to the cell membrane and endosome membrane, consistent with GPCR trafficking dynamics.

• It features seven transmembrane helices, disulfide bonds, and glycosylation, characteristic of rhodopsin-like GPCRs.

• Alternative splicing produces multiple isoforms, potentially modulating ligand affinity and tissue-specific signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal intracellular region (aa 350–384); verify reactivity with your target isoform, especially in samples where alternative splicing occurs.
• For Western blotting, the predicted molecular weight is ~44kDa; glycosylation may cause band shifts—consider deglycosylation controls if needed.
• In ELISA, optimize coating concentration and detection system; the antibody may serve as capture or detection reagent for HTR4 in cell lysates.
• Reported cross-reactivity includes human, mouse, and rat; confirm sequence homology in the immunogen region for other species before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material supply, offering a broad portfolio including validated anti-HTR4 polyclonal antibodies for GPCR and neuroscience research. From monoclonal/polyclonal antibodies to bulk ancillary reagents and custom development, we help diagnostic manufacturers accelerate every stage—from concept to clinic. Contact us to explore sourcing solutions for rare targets and streamlined assay development.