Anti-HTR3A Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P46098

Core Product Specifications and Parameters

Biological Background: HTR3A Function and Localization

• HTR3A encodes the 5-hydroxytryptamine receptor 3A, also known as serotonin receptor 3A, which is a subunit of the serotonin-gated cation-selective channel.
• Upon activation by serotonin, this receptor forms fast, depolarizing responses in neurons, playing a crucial role in synaptic transmission.
• The protein is localized to the postsynaptic cell membrane and is broadly expressed in the cerebral cortex, amygdala, hippocampus, testis, and various peripheral tissues including the intestine and uterus.
• HTR3A is a glycoprotein with disulfide bonds and transmembrane helices, characteristic of the ligand-gated ion channel family.
• Alternative splicing yields multiple isoforms, contributing to functional diversity of the receptor.
• The receptor is involved in ion transport and is a key target for understanding neuropsychiatric disorders and gastrointestinal functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular N-terminal domain (aa 24-241), which is likely to target the ligand-binding region and may be suitable for detecting both glycosylated and unglycosylated forms.
• In Western blot, a band around 55 kDa is expected; however, glycosylation and other modifications may affect mobility. Consider using deglycosylation enzymes if precise molecular weight confirmation is required.
• For immunohistochemistry on paraffin-embedded sections, antigen retrieval with citrate buffer (pH 6.0) is often effective for transmembrane proteins; validate in your tissue of interest.
• This antibody is validated for ELISA; pairing with another antibody targeting a distinct epitope may enhance sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a streamlined one-stop solution for IVD raw-material sourcing. Beyond this anti-HTR3A antibody, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing to accelerate your serotonin receptor-related assay development from concept to clinic.