Anti-HTR2C (5-HT2C) Polyclonal Antibody for WB, ELISA - P28335

Core Product Specifications and Parameters

Biological Background: HTR2C Function and Localization

• HTR2C encodes the 5-hydroxytryptamine receptor 2C (5-HT2C), a G protein-coupled receptor that binds serotonin and mediates its effects.
• Upon serotonin binding, HTR2C couples to G(q)/G(11) alpha proteins and activates phospholipase C-beta, generating second messengers IP3 and DAG, which trigger calcium release and modulate various cellular responses. Related references: PMID:18703043, PMID:29398112
• HTR2C also serves as a receptor for various psychoactive substances, including ergot alkaloids, DOI, and LSD, leading to ligand-dependent signaling. Related references: PMID:19057895, PMID:29398112
• Beta-arrestin family members inhibit G protein-mediated signaling from HTR2C and promote alternative signaling pathways. Related references: PMID:29398112
• HTR2C is involved in regulating appetite, eating behavior, stress responses, and insulin sensitivity, with suggested roles in neuronal activity modulation via transient receptor potential calcium channels.
• The receptor localizes to the cell membrane and is primarily detected in the brain, consistent with its neurological functions.
• Post-translational modifications include glycosylation, disulfide bonds, and RNA editing, contributing to functional diversity.
• HTR2C is alternatively spliced, yielding isoforms with potentially distinct properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 120-220, which may recognize both unmodified and post-translationally modified forms of HTR2C.
• For Western blot, the predicted molecular weight is 52 kDa, but glycosylation and other modifications may cause band shifts; validate in the relevant sample system.
• The sequence homology across human, mouse, and rat in the immunogen region supports cross-reactivity; verify reactivity in your species of interest.
• For ELISA, use as capture or detection antibody; confirm compatibility with your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. This HTR2C antibody supports neurological and pharmacological research applications. For rare target sourcing or custom development, contact CamelBio.