Anti-HTR2B Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P41595

Core Product Specifications and Parameters

Biological Background: HTR2B Function and Localization

• HTR2B, or 5-hydroxytryptamine receptor 2B, is a G protein-coupled receptor that binds serotonin and various ergot alkaloid derivatives, initiating intracellular signaling cascades.
  Related references: PMID:18703043, PMID:23519210, PMID:7926008, PMID:12970106

• Upon ligand binding, HTR2B couples to G(q)/G(11) alpha subunits, activating phospholipase C-beta to generate second messengers DAG and IP3, leading to intracellular Ca²⁺ release.
  Related references: PMID:18703043, PMID:23519215, PMID:28129538

• Beta-arrestin family members interact with HTR2B to promote receptor desensitization and initiate G protein-independent signaling pathways.
  Related references: PMID:23519215, PMID:28129538, PMID:30127358

• HTR2B signaling modulates dopamine and serotonin release and uptake, affecting behavior; genetic variation is linked to impulsive behavior.
  Related references: PMID:21179162

• Critical for embryonic cardiac development: required for normal proliferation of cardiac myocytes and protection against apoptosis.

• Necessary for osteoblast proliferation and bone density maintenance, as well as for the proliferation of interstitial cells of Cajal in the intestine.

• Subcellularly, HTR2B localizes to the cell membrane, synaptic regions, and synaptosomes.

• Ubiquitously expressed in human tissues, with relatively higher levels in liver, kidney, heart, pulmonary artery, and intestine.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal cytoplasmic region (a.a. 382-481) of human HTR2B; therefore, the antibody is expected to recognize the receptor in denaturing Western blot conditions and in immunofluorescence with adequate permeabilization.
• A band at approximately 54 kDa is predicted for human HTR2B; verify expression in relevant tissue or cell lines (e.g., heart, liver, or brain regions) as positive controls.
• The polyclonal nature and recombinant protein immunogen design may provide robust detection across multiple applications; however, optimal dilutions and conditions should be determined experimentally for each assay.
• Cross-reactivity with mouse and rat is reported, making this antibody suitable for rodent model studies; nonetheless, confirm specificity in the species and tissue of interest.
• Because HTR2B shares homology with other 5-HT2 receptor subtypes, potential cross-reactivity should be evaluated using knockout controls or specific inhibitors.
• For ELISA, the antibody can be used as a capture or detection reagent; pairing with a matched antibody pair may enhance sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to high-quality IVD raw materials, including validated antibodies like this anti-HTR2B polyclonal antibody for serotonin receptor studies. Beyond individual reagents, we provide comprehensive solutions for assay development, from antibody pairs and optimized monoclonals to rare target sourcing and ancillary reagents, supporting your projects from concept to clinical application.