Anti-HTR2A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P28223

Core Product Specifications and Parameters

Biological Background: HTR2A Function and Localization

• G protein-coupled receptor for serotonin (5-HT) that also mediates cellular responses to psychoactive substances such as LSD, psilocybin, and mescaline. Related references: PMID:1330647 PMID:18703043 PMID:19057895
• Ligand binding triggers Gq/11-mediated activation of phospholipase C-beta, leading to the production of second messengers diacylglycerol (DAG) and inositol trisphosphate (IP3) that promote intracellular calcium release. Related references: PMID:18703043 PMID:28129538 PMID:35084960
• Beta-arrestin recruitment attenuates G protein signaling and initiates alternative signal transduction cascades. Related references: PMID:28129538 PMID:35084960
• Plays a critical role in the central nervous system, modulating neural activity, perception, cognition, and mood, and is implicated in behavioral responses to anxiety and psychoactive compounds. Related references: PMID:18297054
• Functions as a host cell receptor for human JC polyomavirus (JCPyV), facilitating viral entry.
• Localizes predominantly to the cell membrane and is enriched in neuronal projections, including dendrites, axons, and presynaptic specializations; also detected in cytoplasmic vesicles and caveolae.
• Expressed in brain cortex and blood platelets, with alternative splicing and post-translational modifications including disulfide bond formation, glycosylation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (amino acids 1-100), a region that may help reduce cross-reactivity with other serotonin receptor subtypes; however, validation in specific experimental contexts is advised.
• For Western blot (WB), a band near 53 kDa is expected under reducing conditions; consider molecular weight markers and appropriate gel percentages for resolution.
• In immunofluorescence/ICC, membrane and neurite localization may require optimized fixation and permeabilization protocols; testing with known positive controls (e.g., neuronal cell lines or primary neurons) is recommended.
• The reported cross-reactivity with mouse enables use in rodent models, but confirm performance in your specific tissue or cell type.
• For ELISA applications, consider using the immunizing peptide or recombinant protein as a positive control to validate coating and detection conditions.

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CamelBio provides diagnostic manufacturers, research laboratories, and institutes with one-stop access to high-quality IVD raw materials, including validated antibodies like this anti-HTR2A polyclonal antibody. Our catalog features optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets, supporting neuroscience and signaling research from concept to clinic. Partner with us for tailored supply solutions that streamline your assay development and manufacturing workflows.