Anti-HSPE1/HSP10/CPN10 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P61604

Core Product Specifications and Parameters

Biological Background: HSPE1/HSP10/CPN10 Function and Localization

• HSPE1, also known as chaperonin 10 (CPN10), early-pregnancy factor (EPF), and heat shock protein 10 (HSP10), is a 10 kDa mitochondrial protein encoded by the HSPE1 gene.
• Acts as a co-chaperonin for HSP60 in the mitochondrial matrix, facilitating correct protein folding and import, and preventing misfolding under stress conditions. Related references: PMID:11422376, PMID:1346131, PMID:7912672
• The functional complex comprises two back-to-back heptameric rings of HSP60 capped by heptameric HSP10 rings, creating an isolated folding chamber; ATP hydrolysis drives substrate release.
• Subcellular localization is predominantly the mitochondrial matrix.
• Post-translational modifications include acetylation and phosphorylation, which may regulate chaperone activity.
• Classified under keywords such as chaperone, stress response, and phosphoprotein, highlighting its role in cellular proteostasis.

Experimental Guidance and Technical Tips

• For Western blot, given the small molecular weight (11 kDa), consider using a high-retention PVDF membrane and optimize transfer conditions to avoid protein loss.
• In IHC-P, mitochondrial antigens may require specific antigen retrieval protocols; a pilot experiment with positive control tissue is recommended.
• The antibody cross-reacts with mouse and rat, enabling its use in comparative studies across these species.
• The immunogen covers a conserved central region (aa 50-150), which may account for the observed cross-reactivity; validate specificity in your target species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, supporting your mitochondrial biology and chaperone research needs. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, alongside specialized sourcing for rare targets like HSPE1. Let us help you accelerate development from concept to clinic.