Anti-HSPB8/HSP22 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q9UJY1

Core Product Specifications and Parameters

Biological Background: HSPB8/HSP22 Function and Localization

• HSPB8, also known as heat shock protein beta-8 or HSP22, is a small heat shock protein (sHSP) characterized by an alpha-crystallin domain and functions as an ATP-independent molecular chaperone.
• It is a central component of the chaperone-assisted selective autophagy (CASA) pathway, crucial for the degradation of damaged proteins in mechanically strained tissues such as skeletal and cardiac muscle.
• HSPB8 displays temperature-dependent chaperone activity, effectively preventing stress-induced protein aggregation and maintaining cellular proteostasis.
• Belonging to the HSPB family (small HSPs), HSPB8 shares structural similarities with other family members like HSPB1 and HSPB5, but has unique roles in autophagy-mediated protein quality control.
• Primarily localized to the cytoplasm, HSPB8 can also translocate to the nucleus under certain stress or signaling conditions, as indicated by UniProt annotation.
• Tissue specificity is notable: it is predominantly expressed in skeletal muscle and heart, underscoring its importance in muscle biology and related pathologies.
• Post-translational modifications, including methylation and phosphorylation, regulate its activity. Mutations in HSPB8 are linked to neuropathies such as Charcot-Marie-Tooth disease type 2L (CMT2L) and distal hereditary motor neuropathy (HMN2A), as well as myofibrillar myopathy, highlighting its role in neurodegeneration and muscle disease.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 1–196 of human HSPB8, representing the full-length protein. For Western blotting (WB), expect a band at approximately 22 kDa; validate with appropriate positive controls such as skeletal muscle or heart lysates.
• This polyclonal antibody is suitable for ELISA; optimize coating antigen concentration and detection antibodies for your specific assay format.
• For immunoprecipitation (IP) applications, note that the antibody lacks validated IP data; if pursuing IP, consider using a specific protocol and validating with known interacting partners like BAG3.
• For fluorescence immunostaining (IF-P) and immunohistochemistry (IHC-P), ensure proper fixation and permeabilization conditions to preserve the target epitope, and include relevant isotype controls.
• The antibody cross-reacts with mouse and rat HSPB8; verify species-specific reactivity and specificity in your model system, especially if used in non-human tissues or cell lines.
• When performing multiple applications, note that optimal dilutions and conditions may vary; conduct pilot experiments to establish the best working parameters for your research.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a reliable partner for diagnostic manufacturers and research laboratories, offering one-stop access to IVD raw materials, technical services, and consulting. We supply validated antibody pairs, optimized polyclonal antibodies like this anti-HSPB8/HSP22 antibody, and bulk ancillary reagents to support projects in muscle biology, protein quality control, and neurodegeneration research. Count on CamelBio for seamless sourcing from discovery through clinical application.