Anti-HSPB8/HSP22 Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q9UJY1

Core Product Specifications and Parameters

Biological Background: HSPB8/HSP22 Function and Localization

• HSPB8, also known as HSP22, alpha-crystallin C chain, and E2-induced gene 1 protein, is a member of the small heat shock protein (sHSP) family.
• Functions as a temperature-dependent molecular chaperone involved in chaperone-assisted selective autophagy (CASA), essential for protein quality control, especially in mechanically stressed tissues like muscle.
• Localizes to both the cytoplasm and nucleus.
• Predominantly expressed in skeletal muscle and heart tissue.
• Post-translational modifications include phosphorylation and methylation, as indicated by proteomics identification.
• Genetic variations in HSPB8 are associated with neuropathies such as Charcot-Marie-Tooth disease and myofibrillar myopathy, underscoring its role in neurodegeneration.
• UniProt keywords: Chaperone, Stress response, Disease variant, Neuropathy.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human HSPB8 sequence (aa 1-196), so the antibody is likely to recognize the native protein; for applications like IF and IHC, consider optimizing fixation and permeabilization conditions.
• For WB, a band around 22 kDa is expected; validate using positive controls from muscle or heart tissue lysates.
• Given cross-reactivity with mouse, rat, and monkey, the antibody may be suitable for studies across these species; however, it is advisable to confirm reactivity in the relevant sample system.
• As a monoclonal antibody, it can offer consistent lot-to-lot performance; store at recommended temperatures and avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research teams with a comprehensive catalog of IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies. This anti-HSPB8/HSP22 antibody supports research into protein quality control and chaperone-assisted autophagy, particularly in muscle and neurodegenerative disease models. For bulk orders, ancillary reagents, or assistance in sourcing rare target antibodies, our team provides end-to-end procurement solutions from concept to clinic.