Anti-HSPB7 Polyclonal Antibody for WB, IHC-P, ELISA - Q9UBY9

Core Product Specifications and Parameters

Biological Background: Heat shock protein beta-7 Function and Localization

• Heat shock protein beta-7 (HSPB7), also known as cardiovascular heat shock protein (cvHSP), is a member of the small heat shock protein (sHSP) family.
• The HSPB7 gene undergoes alternative splicing, producing at least three isoforms; isoform 1 is the predominant form detected in heart and skeletal muscle.
• Isoform 1 localizes to the cytoplasm and nucleus, with enrichment in Cajal bodies, suggesting roles in nuclear RNA metabolism or stress responses.
• Tissue expression is largely restricted to cardiac and skeletal muscle, with very low levels in adipose tissue and aorta; other isoforms are poorly expressed in heart.
• Annotated keywords include chaperone and stress response, indicating a likely role in maintaining protein homeostasis under cellular stress.
• The protein has a molecular weight of approximately 18.6 kDa (predicted from sequence) and has been identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-170), which may target all isoforms; consider sequence alignment if isoform-specific detection is required.
• For Western blot, the predicted molecular weight is ~19 kDa; verify band size in relevant tissue lysates such as heart or skeletal muscle.
• For IHC-P, optimize antigen retrieval and titrate antibody concentration due to tissue-specific expression patterns.
• ELISA performance may vary; validate using recombinant protein or peptide as coating antigen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-HSPB7 polyclonal antibody supports cardiovascular disease and stress response research. Our supply network includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets like HSPB7.