Anti-HSPB2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q16082

Core Product Specifications and Parameters

Biological Background: HSPB2 Function and Localization

• Heat shock protein beta-2 (HSPB2), also known as DMPK-binding protein (MKBP), is a member of the small heat shock protein family.
• The protein may regulate the serine/threonine kinase DMPK, though its precise role in stress responses is not fully characterized.
• HSPB2 localizes to both the cytoplasm and nucleus, suggesting dynamic spatial regulation.
• It is preferentially expressed in skeletal muscle and heart, and is not detected in the lens.
• Structural studies (3D-structure) are available for HSPB2, providing insights into its chaperone-like function.
• The protein is associated with stress response and has been identified in proteomics screens.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human HSPB2 (amino acids 1-182), supporting broad epitope recognition.
• For Western blot, consider using human skeletal muscle or heart lysates to validate endogenous HSPB2 detection; mouse samples are also suitable given cross-reactivity.
• In immunofluorescence/ICC, anticipate both cytoplasmic and nuclear staining patterns.
• ELISA applications can employ the recombinant immunogen as a standard to calibrate detection sensitivity.
• As a polyclonal antibody, lot-to-lot consistency in application-specific performance should be confirmed in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for diagnostic manufacturers and researchers by offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-HSPB2 antibody fits into stress response, kinase regulation, and muscle biology workflows. Contact us to build a complete raw-material solution tailored to your assay development pipeline.