Anti-HSPA6 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P17066

Core Product Specifications and Parameters

Biological Background: HSPA6 Function and Localization

• HSPA6, also known as heat shock 70 kDa protein B' (HSP70B'), is a member of the heat shock protein 70 (HSP70) family encoded by the HSPA6 gene in humans.
• It functions as a molecular chaperone that plays a critical role in protein quality control, assisting in the correct folding of newly synthesized polypeptides, refolding of misfolded proteins, and targeting of damaged proteins for degradation.
• The chaperone activity is regulated by ATP binding and hydrolysis. ATP-bound HSPA6 has low substrate affinity, while ADP-bound form has high affinity, enabling cycles of substrate binding and release.
• Related references: PMID:26865365
• HSPA6 is involved in the cellular stress response, protecting the proteome under adverse conditions and contributing to the maintenance of protein homeostasis.
• Its functional roles extend to the activation of proteolysis, formation and dissociation of protein complexes, and transport of proteins within the cell.
• The protein is subject to methylation as a post-translational modification, although the precise functional impact of this modification on HSPA6 is not detailed in the current record.
• HSPA6 is classified under the keywords ATP-binding, nucleotide-binding, and stress response, reflecting its core biochemical activities and physiological roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 474-643) of human HSPA6; the antibody may recognize both native and denatured forms of the protein.
• For Western blot (WB), expect a specific band near 71 kDa; include appropriate positive and negative controls to validate specificity.
• In immunohistochemistry (IHC-P), optimization of antigen retrieval and antibody dilution is recommended for each sample type.
• For ELISA, the antibody may be used as a capture or detection reagent; perform checkerboard titrations to determine optimal working concentrations.
• Cross-reactivity with mouse and rat HSPA6 is declared; verify reactivity in the relevant species and tissue systems as needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for high-quality IVD raw materials, offering validated antibodies like this anti-HSPA6 polyclonal antibody along with complementary reagents and technical support. We specialize in bridging supply gaps for heat shock protein research and stress response diagnostic development. Our portfolio includes optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare target raw materials to streamline your workflow from concept to clinic.