Anti-HSPA4L Polyclonal Antibody for WB, IF/ICC, ELISA - O95757

Core Product Specifications and Parameters

Biological Background: HSPA4L Function and Localization

• HSPA4L (Heat shock 70 kDa protein 4L), also known as APG-1, HSPH3, and Osp94, belongs to the heat shock protein 70 (HSP70) family.
• It encodes a ~95 kDa protein with a predicted molecular weight of 94,512 Da.
• HSPA4L functions as a molecular chaperone, demonstrating in vitro activity by inhibiting the aggregation of citrate synthase.
• The protein contains an ATP-binding domain, consistent with its classification as a nucleotide-binding chaperone.
• HSPA4L localizes to the cytoplasm and nucleus.
• It is identified as a phosphoprotein (by proteomics), suggesting regulation by phosphorylation.
• The protein is part of the reference proteome and is associated with stress response pathways.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment covering amino acids 500–600; the antibody likely recognizes a linear epitope, making it well-suited for denaturing Western blot applications.
• Consider inducing cellular stress (e.g., heat shock) to upregulate HSPA4L expression when performing IF/ICC or WB on samples with low basal expression.
• Cross-reactivity with mouse and rat allows this antibody to be used in rodent model studies; validate in the relevant species-specific tissue or cell line.
• For ELISA, the antibody can be used to detect recombinant HSPA4L or as a capture/detection pair component; standard optimization of coating and detection conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material procurement, offering a comprehensive range of high-quality antibodies, antigens, and auxiliary reagents for diagnostic development. This HSPA4L polyclonal antibody targets a conserved stress-response chaperone, and we can further support your needs with validated antibody pairs, bulk monoclonal or polyclonal antibodies, and specialized raw materials for heat shock protein research. From concept to clinic, streamline your sourcing with CamelBio’s integrated supply chain and expert consulting.