Anti-HSPA4 Rabbit Polyclonal Antibody for WB, ELISA - P34932

Core Product Specifications and Parameters

Biological Background: HSPA4 Function and Localization

• HSPA4, also known as heat shock 70 kDa protein 4, is a molecular chaperone belonging to the HSP70 family. Its UniProt keywords include 'Stress response', 'ATP-binding', and 'Nucleotide-binding', indicating involvement in cellular stress adaptation and protein homeostasis.
• Alternative names for HSPA4 are HSP70RY, APG-2, and heat shock protein family H member 2, reflecting its historical or functional classifications.
• The protein is localized to the cytoplasm, as annotated by 'Cytoplasm' in subcellular location and keyword fields.
• Post-translational modifications documented include acetylation, phosphorylation, and methylation, identified through direct protein sequencing and proteomics approaches.
• Alternative splicing generates multiple isoforms of HSPA4, adding to the functional diversity of the protein.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 680–840 of human HSPA4; consider this region for possible blocking peptide experiments or when designing complementary detection reagents.
• For Western blot, a band around 94 kDa is expected; validate detection in the relevant sample system, taking into account cross-reactivity with human, mouse, and rat.
• In ELISA, titrate the antibody under appropriate conditions, and include proper controls to confirm specificity against the target protein.
• Because HSPA4 is a stress-responsive chaperone, consider analyzing expression changes under heat shock or other stress conditions to confirm biological relevance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to supply a comprehensive range of IVD raw materials, including validated antibodies, optimized polyclonal and monoclonal reagents, bulk ancillary proteins, and rare-target sourcing solutions. For HSPA4 and other stress-related chaperones, we offer seamless access to high-quality antibody products and technical support, helping you streamline your research and development pipeline from concept to clinic.