Anti-HSPA4 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P34932

Core Product Specifications and Parameters

Biological Background: HSPA4 Function and Localization

• HSPA4 (Heat shock 70 kDa protein 4) is also known as HSP70RY, APG-2, and heat shock protein family H member 2. It is encoded by the HSPA4 gene (also referred to as APG2 or HSPH2) on human chromosome 5.
• As a member of the Hsp70 family, HSPA4 features a highly conserved ATPase domain and a substrate-binding domain, enabling it to function as an ATP-dependent molecular chaperone involved in protein folding and stress response.
• The protein contains nucleotide-binding motifs, consistent with its ATP-binding capability, which is essential for its chaperone cycle and interaction with co-chaperones.
• Subcellular localization studies indicate that HSPA4 is predominantly cytoplasmic, where it exerts its chaperone functions under both normal and stress conditions.
• Post-translational modifications of HSPA4 include acetylation, phosphorylation, and methylation, which may regulate its activity, stability, or protein-protein interactions.
• Alternative splicing of the HSPA4 transcript gives rise to multiple isoforms, potentially expanding its functional diversity in different cellular contexts.
• HSPA4 is classified as a stress response protein, and its expression is likely upregulated under conditions of cellular stress to prevent protein aggregation and maintain proteostasis.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a synthetic peptide spanning amino acids 700–800 of human HSPA4 (P34932). This region resides in the C-terminal domain, which is less conserved among Hsp70 family members and may confer target specificity.
• For Western blot (WB) applications, consider using a standard denaturing SDS-PAGE protocol with a protein ladder covering the 94 kDa range. Optimize transfer conditions for high-molecular-weight proteins and validate detection with appropriate positive controls (e.g., HeLa or HEK293 lysates).
• In immunohistochemistry (IHC-P), antigen retrieval methods (e.g., heat-induced epitope retrieval at pH 6 or 9) and antibody dilutions should be empirically optimized for each tissue type to reduce background and achieve specific staining.
• For ELISA, ensure the coating antigen and detection antibody pair have been thoroughly tested and perform checkerboard titrations to establish optimal working concentrations.
• Because this antibody is raised against a peptide immunogen, it may recognize linear epitopes exposed under denaturing conditions (e.g., WB) but may need validation in native conformational assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, laboratories, and research institutes with bulk IVD raw materials, including validated antibody pairs, monoclonal antibodies, and ancillary reagents. This anti-HSPA4 monoclonal antibody supports research in cellular stress responses and protein folding, making it a reliable choice for users developing assays in these areas. Beyond this product, CamelBio offers comprehensive sourcing support for rare targets and one-stop procurement solutions—from concept to clinic.