Anti-HSPA2 Monoclonal Antibody for WB, IF/ICC, ELISA - P54652

Core Product Specifications and Parameters

Biological Background: HSPA2 Function and Localization

• Protein identity: HSPA2, also known as Heat shock-related 70 kDa protein 2, heat shock protein family A member 2, HSP70-2, and HSP70-3, is a member of the HSP70 family.
• Chaperone function: Acts as a molecular chaperone in protein quality control, assisting in folding, refolding, and degradation of proteins through cycles of ATP binding, hydrolysis, and ADP release, regulating substrate affinity (PubMed:26865365). Related references: PMID:26865365
• Subcellular localization: Found in the cytoplasm and associates with the cytoskeleton, particularly the spindle during meiosis.
• Spermatogenesis role: Plays a crucial role in spermatogenesis; in association with SHCBP1L, may maintain spindle integrity during meiosis in male germ cells (By similarity).
• Post-translational modifications: Subject to methylation and phosphorylation as indicated by keywords.
• Stress response: Induced under stress conditions to protect the proteome, aligning with its keyword classification as a stress response protein.
• ATP-binding and nucleotide-binding: Contains ATP-binding domains essential for its chaperone cycle, as supported by structural data (3D-structure keyword).

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against recombinant protein spanning amino acids 488-637, covering part of the C-terminal region. For assays targeting specific isoforms or requiring native conformation, verify the antibody's performance under your experimental conditions.
• Application notes: Suitable for WB, IF/ICC, IF-P, and ELISA. Consider using heat-shocked cells or testis-derived samples as positive controls, given HSPA2's expression profile and stress-inducibility.
• Species cross-reactivity: Detects human, mouse, and rat HSPA2. If working with other species, perform a sequence alignment to evaluate potential cross-reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material solutions, connecting diagnostic manufacturers and research laboratories with high-quality reagents from concept to clinic. This anti-HSPA2 monoclonal antibody exemplifies our capability to supply optimized antibodies for chaperone biology, stress response, and spermatogenesis research. Beyond individual antibodies, we provide validated antibody pairs, bulk ancillary reagents, and specialized sourcing for rare-target raw materials to accelerate your assay development and commercialization.