Anti-HSPA14 Rabbit Polyclonal Antibody for WB, ELISA - Q0VDF9

Core Product Specifications and Parameters

Biological Background: HSPA14 Function and Localization

• HSPA14, also known as HSP70-like protein 1, HSP70-4, HSP70L1, MSANTD7, and heat shock protein HSP60, is encoded by the HSPA14 gene (Gene ID: 51182) and is a member of the heat shock protein 70 (HSP70) family.
• It functions as a component of the ribosome-associated complex (RAC), which plays a critical role in maintaining nascent polypeptide chains in a folding-competent state during translation.
• In the RAC, HSPA14 directly binds to nascent polypeptides, while its ATPase activity is stimulated by the co-chaperone DNAJC2, facilitating proper protein folding.
• The protein is localized to the cytoplasm and cytosol, consistent with its role in co-translational protein folding.
• HSPA14 contains ATP-binding and nucleotide-binding domains, characteristic of molecular chaperones that rely on ATP hydrolysis for their activity.
• It has been identified through proteomics approaches (UniProt keyword: Proteomics identification), highlighting its relevance in large-scale protein expression studies.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant fusion protein spanning amino acids 240-509 of human HSPA14 (NP_057383.2), which covers part of the substrate-binding domain; consider using standard blocking and washing conditions for western blotting.
• For ELISA applications, titrate the antibody to determine the optimal working concentration and include appropriate positive and negative controls.
• Given its predicted molecular weight of 55 kDa, verify protein migration using appropriate molecular weight markers and validate the band size in relevant human or mouse sample lysates.
• As with any polyclonal antibody, batch-to-batch consistency should be tested for critical quantitative assays, and it is advisable to use the antibody in conjunction with validated controls specific to the experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibodies for heat shock proteins and protein folding research. For your HSPA14 studies, we provide bulk quantities of this rabbit polyclonal antibody along with ancillary reagents and customized sourcing services. From concept to clinic, CamelBio supports diagnostic manufacturers and researchers with reliable supply chains and technical expertise.