Anti-HSPA12A Monoclonal Antibody for WB and ELISA - O43301

Core Product Specifications and Parameters

Biological Background: HSPA12A Function and Localization

• HSPA12A (heat shock 70 kDa protein 12A) is a member of the heat shock protein 70 (HSP70) family, encoded by the HSPA12A gene in humans.
• The protein serves as an adapter for SORL1 (sortilin-related receptor 1), modulating its subcellular localization and delaying its internalization; it does not interact with SORT1.
• HSPA12A is widely expressed across tissues, with the highest levels observed in the brain, kidney, and skeletal muscle.
• Subcellularly, the protein localizes to both the cytoplasm and the nucleus, consistent with its role in intracellular trafficking.
• The protein contains an ATP-binding domain, characteristic of the HSP70 family, and is subject to post-translational acetylation.
• With a molecular weight of approximately 75 kDa, HSPA12A is identified as a reference proteome component in comprehensive proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide spanning amino acids 50–150 of human HSPA12A; this region is likely exposed for antibody recognition in native and denatured conditions.
• For Western blot, consider starting with a 1:500–1:2000 dilution of the antibody and validate in the relevant sample system; the predicted band is ~75 kDa.
• In ELISA applications, optimize the coating antigen and antibody concentrations to achieve optimal signal-to-noise ratio.
• Cross‑reactivity with mouse and rat HSPA12A is expected due to sequence similarity; verify detection in your specific experimental context.
• Always include appropriate positive and negative controls, such as cell lines with known HSPA12A expression or siRNA‑mediated knockdown.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, laboratories, and research institutes with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This HSPA12A antibody supports studies on intracellular trafficking and stress response pathways, and CamelBio can assist in sourcing related reagents or developing custom solutions for your assay development. As a one‑stop bridge from concept to clinic, CamelBio streamlines your procurement of rare‑target raw materials and offers technical consulting to accelerate your diagnostic projects.