Anti-Hsp90β Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P08238

Core Product Specifications and Parameters

Biological Background: Hsp90β Function and Localization

• Hsp90β (gene HSP90AB1) is a constitutively expressed member of the heat shock protein 90 family, functioning as an ATP-dependent molecular chaperone essential for the maturation and stability of numerous client proteins involved in cell cycle control and signal transduction.
• It undergoes a conformational cycle coupled to ATP hydrolysis, regulated by co-chaperones like p23 and Aha1, which modulate substrate binding and release (PubMed:16478993, PubMed:19696785). Related references: PMID:16478993 PMID:19696785
• Hsp90β facilitates proper folding of kinases and transcription factors, and also plays a direct role in transcription regulation by modulating epigenetic modifiers and evicting histones from gene promoters (PubMed:25973397). Related references: PMID:25973397
• In TGF-beta signaling, Hsp90β antagonizes STUB1-mediated Smad3 ubiquitination and degradation, thereby promoting signaling (PubMed:24613385). Related references: PMID:24613385
• The chaperone supports cell differentiation by protecting BIRC2 (cIAP1) from auto-ubiquitination and proteasomal degradation (PubMed:18239673). Related references: PMID:18239673
• Under heat shock, Hsp90β chaperones JAK2 and PRKCE to mediate STAT1 phosphorylation and activation, which in turn upregulates its own expression (PubMed:20353823). Related references: PMID:20353823
• Hsp90β is implicated in the secretion of leaderless proteins like IL-1β via interaction with the cargo receptor TMED10 at the ER-Golgi intermediate compartment (PubMed:32272059). Related references: PMID:32272059
• Subcellular localization includes cytoplasm, nucleus, melanosome, cell membrane, and it can be secreted; subject to numerous post-translational modifications such as acetylation, phosphorylation, methylation, and S-nitrosylation.

Experimental Guidance and Technical Tips

• For Western blotting, a band at approximately 83 kDa is expected; validate with appropriate controls, including knockout cell lines given the KO validated designation.
• For immunohistochemistry (IHC-P), consider antigen retrieval methods and test on known positive tissues such as tumor samples or heat-shocked cells.
• For ELISA, optimize coating antigen and antibody concentration, and consider using paired antibodies for sandwich ELISA development.
• Given the polyclonal nature, expect batch-to-batch variability; it is advisable to reserve aliquots of the same batch for consistent results across experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated partner for IVD raw material procurement, connecting diagnostic manufacturers and research laboratories with high-quality reagents and technical expertise. This anti-Hsp90β antibody supports investigations into molecular chaperone biology, cancer signaling, and stress response pathways. Explore our extensive inventory for validated antibody pairs, bulk ancillary reagents, and customized sourcing of rare targets to accelerate your assay development from concept to clinical application.