Anti-Hsp90β Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P08238

Core Product Specifications and Parameters

Biological Background: Hsp90β Function and Localization

• HSP90-beta is a molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved in cell cycle control and signal transduction. (PubMed:16478993, PubMed:19696785) Related references: PMID:16478993 PMID:19696785
• It undergoes an ATPase-driven functional cycle that induces conformational changes in client proteins, interacting with various co-chaperones for substrate recognition and processing. (PubMed:26991466, PubMed:27295069) Related references: PMID:26991466 PMID:27295069
• Beyond chaperoning, HSP90-beta regulates transcription by altering transcription factor steady-state levels, modulating epigenetic modifiers, and participating in histone eviction from promoter regions to activate gene expression. (PubMed:25973397) Related references: PMID:25973397
• It antagonizes STUB1-mediated inhibition of TGF-beta signaling by protecting SMAD3 from ubiquitination and degradation. (PubMed:24613385) Related references: PMID:24613385
• Main chaperone involved in the phosphorylation/activation of STAT1 under heat shock, by chaperoning both JAK2 and PRKCE, and in turn activates its own transcription. (PubMed:20353823) Related references: PMID:20353823
• (Microbial infection) Binding to Neisseria meningitidis NadA stimulates monocytes and may interfere with NadA-mediated invasion of human cells. (PubMed:21949862) Related references: PMID:21949862
• Subcellular localization includes cytoplasm, melanosome, nucleus, secreted, cell membrane, dynein axonemal particle, and cell surface.
• Post-translational modifications include acetylation, phosphorylation, methylation, S-nitrosylation, and ubiquitin conjugation, reflecting its regulatory complexity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-300) of human Hsp90β, encompassing the ATP-binding domain; the antibody is expected to recognize total Hsp90β.
• For Western blot, a band at approximately 83 kDa is anticipated; validate detection in your sample system due to potential post-translational modifications or isoforms.
• In immunohistochemistry (IHC-P), perform appropriate antigen retrieval and test antibody dilution; consider controls to verify specificity.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization conditions to preserve both morphology and antigen accessibility.
• ELISA applications may require pairwise testing with matched antibody pairs; assess sensitivity with recombinant Hsp90β protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support diagnostic manufacturers and research laboratories. Our anti-Hsp90β rabbit monoclonal antibody caters to studies on chaperone biology, heat shock response, and cancer diagnostics. With comprehensive supply capabilities from concept to clinic, CamelBio streamlines your development pipeline.