Anti-Hsp90α/β Rabbit Monoclonal Antibody for WB, ELISA - P07900 / P08238

Core Product Specifications and Parameters

Biological Background: Hsp90α/β Function and Localization

• Encodes Heat shock protein HSP 90-alpha, a molecular chaperone crucial for protein folding and activation. Alternative names include HSP86, LAP-2, and HSP90A.
• Functions as an ATP-dependent chaperone, promoting maturation, structural maintenance, and regulation of client proteins involved in cell cycle control and signal transduction. Related references: PMID:11274138, PMID:12526792, PMID:15577939
• Interacts dynamically with co-chaperones that modulate substrate recognition; ATP binding and hydrolysis drive conformational changes essential for chaperone activity. Related references: PMID:29127155, PMID:26991466, PMID:27295069
• Plays a critical role in mitochondrial import by delivering preproteins to the mitochondrial import receptor TOMM70. Related references: PMID:12526792
• Modulates transcription at multiple levels: alters transcription factor stability, regulates epigenetic modifiers, and participates in histone eviction from promoters. Related references: PMID:25973397
• Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion; also promotes host antiviral response via TOMM70 association with IRF3/TBK1. Related references: PMID:11276205, PMID:24613385, PMID:20628368, PMID:25609812
• Subcellular localization includes nucleus, cytoplasm, melanosome, cell membrane, and mitochondrion, reflecting its multifaceted chaperone functions.
• Post-translational modifications such as acetylation, phosphorylation, S-nitrosylation, and ubiquitination regulate its activity and interactions. The antibody also recognizes Hsp90β (P08238) due to high sequence homology, enabling detection in human, mouse, rat, and monkey samples.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fusion protein corresponding to amino acids 533-732 of human Hsp90α. Validate its performance in your specific sample system, as endogenous Hsp90 may require optimized conditions.
• Predicted molecular weight is ~90 kDa; in Western blotting, consider the possibility of post-translational modifications or isoforms that may alter migration.
• This antibody cross-reacts with human, mouse, rat, and monkey; for other species, check sequence homology of the immunogen region.
• For ELISA, pair with a suitable detection antibody and validate using recombinant protein or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop supplier for IVD raw materials, CamelBio provides reliable and validated antibody pairs, monoclonal antibodies, and bulk ancillary reagents to support your Hsp90 research in chaperone biology, oncology, and stress response. Whether you need optimized antibodies for WB/ELISA or require assistance sourcing rare targets, our team offers tailored procurement solutions to accelerate your diagnostic development from concept to clinic.