Anti-HSP70/HSPA1A Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P0DMV8/P0DMV9

Core Product Specifications and Parameters

Biological Background: HSP70/HSPA1A Function and Localization

• HSPA1A (Heat Shock 70 kDa Protein 1A) is a canonical member of the HSP70 family of molecular chaperones, essential for protein homeostasis under both normal and stress conditions (UniProt).
• Acts as an ATP-dependent chaperone: it cycles between ATP-bound (low substrate affinity) and ADP-bound (high substrate affinity) states, facilitating substrate binding and release, with J-domain co-chaperones (HSP40s) stimulating ATPase activity and nucleotide exchange factors (BAG family) promoting ADP release. Related references: PMID:24012426 PMID:24318877 PMID:26865365
• Governs protein triage: its acetylation status dictates whether it promotes refolding via HOPX or ubiquitin‑mediated degradation via STUB1/CHIP, thereby balancing proteome quality control. Related references: PMID:27708256
• Localizes primarily to the cytoplasm and nucleus, but also associates with the centrosome during mitosis, where it is required for spindle pole integrity and bipolar spindle assembly. Related references: PMID:27137183
• Regulates key signaling pathways: enhances STUB1‑mediated ubiquitination and degradation of SMAD3 (inhibiting TGF‑β signaling) and FOXP3 (in regulatory T cells during inflammation). Related references: PMID:24613385 PMID:23973223
• Participates in the clearance of misfolded transcription factors, such as PRDM1/Blimp‑1, by retaining them in the cytoplasm and targeting them for proteasomal degradation. Related references: PMID:28842558
• Negatively regulates HSF1 transcriptional activity during the recovery phase of the heat shock response, contributing to attenuation of the stress response. Related references: PMID:9499401
• Can be secreted extracellularly and, in certain pathological contexts, serves as a host cell receptor for rotavirus A entry. (SWISS-PROT)

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 500-600 of human HSPA1A, a region that is highly conserved across human, mouse, and rat; thus, broad cross‑reactivity is expected, but users should validate performance in their specific sample system.
• For Western blot, a band at ~70 kDa is observed; always include appropriate positive (e.g., heat‑shocked HeLa lysate) and negative controls, and consider using a housekeeping protein for normalization.
• For immunofluorescence and immunohistochemistry, optimize fixation and permeabilization conditions, as HSP70 can be present in both cytoplasmic and nuclear compartments; antigen retrieval may be necessary for formalin‑fixed paraffin‑embedded sections.
• When using in ELISA, ensure the coating buffer and blocking conditions are compatible with polyclonal antibodies to minimize non‑specific binding.
• This antibody has been reported for use in Western blot, immunofluorescence/immunocytochemistry, immunohistochemistry (paraffin), and ELISA; for other applications, further optimization may be required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high‑quality IVD raw materials, including validated antibodies like this anti‑HSP70 polyclonal antibody. Our portfolio spans a wide range of chaperone and stress‑response targets, supported by complementary reagents such as recombinant proteins, ELISA kits, and custom antibody development services. From concept to clinic, we help accelerate your assay development with reliable bulk supply and technical consultation.