Anti-HSP70 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P0DMV8 / P0DMV9

Core Product Specifications and Parameters

Biological Background: HSP70 Function and Localization

• HSP70, encoded by the HSPA1A (P0DMV8) and HSPA1B (P0DMV9) genes, is a highly conserved molecular chaperone belonging to the heat shock protein 70 family. It is also known as HSP72, HSP70-1, HSP70.1, and stress-inducible HSP70.
• Functions as an ATP-dependent chaperone that cycles between low substrate affinity (ATP-bound) and high affinity (ADP-bound) states, regulated by co-chaperones such as HSP40s, nucleotide exchange factors (e.g., BAG family), and TPR domain proteins (e.g., HOPX, STUB1). Related references: PMID:24012426, PMID:24318877, PMID:26865365
• Acetylation status determines functional bias: acetylated HSP70 binds HOPX to promote protein refolding, while deacetylated HSP70 recruits the ubiquitin ligase STUB1 to target substrates for proteasomal degradation. Related references: PMID:27708256
• Required for the maintenance of functional mitotic centrosomes and proper assembly of the bipolar spindle. Related references: PMID:27137183
• Enhances STUB1-mediated ubiquitination and degradation of SMAD3, thereby facilitating the inhibition of TGF-beta signaling. Related references: PMID:24613385
• Essential for the STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells during inflammation. Related references: PMID:23973223
• Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase of the heat shock response. Related references: PMID:9499401
• Predominantly cytoplasmic but also localizes to the nucleus and centrosome; can be secreted extracellularly.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band at approximately 70 kDa; consider using heat-shocked HeLa or Jurkat cell lysates as positive controls.
• In immunohistochemistry (paraffin), optimize antigen retrieval conditions (e.g., citrate buffer pH 6.0, heat-induced) and titrate the antibody for specific staining.
• Cross-reactivity with mouse and rat HSP70 allows multi-species studies; however, validate performance in your specific experimental model.
• The immunogen lies within amino acids 500–600 of human HSP70, which may influence epitope accessibility in certain applications.
• As a recombinant rabbit monoclonal antibody, this product offers high batch-to-batch reproducibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials. This Anti-HSP70 Rabbit mAb is part of our extensive antibody collection targeting key chaperone and stress response proteins, suitable for development of immunoassays and research kits. Beyond single antibodies, we offer validated antibody pairs, conjugated reagents, and customized bulk production to streamline your assay development pipeline.