Anti-HSP70 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P0DMV8 / P0DMV9

Core Product Specifications and Parameters

Biological Background: HSP70 (HSPA1A/HSPA1B) Function and Localization

• Heat shock 70 kDa protein 1A (HSPA1A) and 1B (HSPA1B) are highly homologous molecular chaperones involved in protein folding, trafficking, and degradation.
• Acts as an ATP-dependent chaperone, cycling between low-affinity (ATP-bound) and high-affinity (ADP-bound) states for substrate proteins, regulated by co-chaperones including J-domain proteins, nucleotide exchange factors (BAG family), and TPR domain chaperones like HOPX and STUB1. Related references: PMID:24012426, PMID:24318877, PMID:26865365
• Acetylation/deacetylation switches HSP70 function between protein refolding (acetylated, binds HOPX) and degradation (deacetylated, binds STUB1/CHIP). Related references: PMID:27708256
• Maintains proteostasis during stress; enhances STUB1-mediated ubiquitination of signaling proteins SMAD3 and FOXP3, modulating TGF-beta and Treg pathways. Related references: PMID:24613385, PMID:23973223
• Required for centrosome integrity and bipolar spindle assembly during mitosis. Related references: PMID:27137183
• Negatively regulates HSF1 transcriptional activity to attenuate heat shock response. Related references: PMID:9499401
• Clears misfolded proteins like PRDM1 via SYNV1/HRD1‑mediated proteasomal degradation. Related references: PMID:28842558
• (Microbial infection) Functions as a post-attachment receptor for rotavirus A entry.
• Subcellularly located in cytoplasm, nucleus, centrosome, and can be secreted; post-translationally modified by acetylation, methylation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 500-600 of human HSP70 (NP_005336.3), a region conserved across species, supporting cross-reactivity with human, mouse, rat, wheat, and maize.
• Suitable for Western blot (WB), immunohistochemistry (IHC-P), immunofluorescence (IF/ICC), and ELISA. Optimize antibody dilution for each assay and sample type.
• For WB, the expected band size is approximately 70 kDa; include appropriate positive and negative controls.
• Validate in the relevant cell or tissue system, especially under heat shock or stress conditions that upregulate HSP70.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This HSP70 antibody supports research and diagnostic applications in protein quality control, cellular stress, and cancer biology. Our supply platform ensures reliable access to rare-target raw materials, bridging the gap from concept to clinical use.