Anti-HSP60/HSPD1 Monoclonal Antibody (Rabbit mAb) for WB, IHC-P, IF/ICC, ELISA - P10809

Core Product Specifications and Parameters

Biological Background: HSP60/HSPD1 Function and Localization

• HSP60, encoded by the HSPD1 gene, is a 60 kDa mitochondrial chaperonin also known as Chaperonin 60 (CPN60), heat shock protein 60 (HSP-60), and HuCHA60. It belongs to the chaperonin family and functions as a key protein folding catalyst.
• Within the mitochondrial matrix, HSP60 cooperates with its co-chaperonin Hsp10 to facilitate correct folding of newly imported proteins, preventing misfolding and promoting refolding under stress conditions. Related references: PMID:11422376, PMID:1346131
• The functional chaperonin complex comprises two heptameric rings of HSP60 that form a back-to-back double-ring structure. Substrate protein binds one ring, followed by ATP binding and association with two heptameric Hsp10 rings, creating an encapsulated folding chamber.
• Subcellularly, HSP60 is localized exclusively to the mitochondrial matrix, where it ensures proper protein homeostasis and mitochondrial function.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin conjugation, and alternative splicing produces isoforms. Mutations in HSPD1 are linked to neurodegenerative disorders such as hereditary spastic paraplegia and leukodystrophy.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide; therefore, the antibody likely recognizes a linear epitope. Optimize antigen retrieval for IHC-P and fixation conditions for IF/ICC accordingly.
• In Western blot, expect a band at approximately 61 kDa. Use appropriate positive and negative controls (e.g., mitochondrial extracts) and consider siRNA or knockout validation to confirm specificity.
• For IHC-P and IF/ICC, pre-adsorption with the immunogen peptide can help verify signal specificity.
• Cross-reactivity is reported for human, mouse, rat, and wheat, but independent verification in your sample type is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with reliable access to IVD raw materials, including validated monoclonal antibodies like the HSP60/HSPD1 Rabbit mAb, optimized for applications in mitochondrial biology and protein folding research. As your sourcing partner, we offer comprehensive supply solutions—from validated antibody pairs and bulk ancillary reagents to rare target sourcing—supporting your development from concept to clinic. Contact us to streamline your procurement and accelerate your diagnostic assay development.